The leading edge (∼1 μm) of lamellipodia in Xenopus laevis keratocytes and fibroblasts was
shown to have an extensively branched organization of actin filaments, which we term the …

While the protrusive event of cell locomotion is thought to be driven by actin polymerization,
the mechanism of forward translocation of the cell body is unclear. To elucidate the …

To track the behavior of human immunodeficiency virus (HIV)-1 in the cytoplasm of infected
cells, we have tagged virions by incorporation of HIV Vpr fused to the GFP. Observation of …

The reconstitution of microbial rocketing motility in vitro with purified proteins has recently
established definitively that no myosin motor is required for protrusion. Instead, actin …

Afilopodium protrudes by elongation of bundled actin filaments in its core. However, the
mechanism of filopodia initiation remains unknown. Using live-cell imaging with GFP-tagged …

Cell motility requires lamellipodial protrusion, a process driven by actin polymerization. Ena/VASP
proteins accumulate in protruding lamellipodia and promote the rapid actin-driven …

The actin cytoskeleton—a collection of actin filaments with their accessory and regulatory
proteins—is the primary force-generating machinery in the cell. It can produce pushing (…

Understanding how a particular cell type expresses the lamellipodial or filopodial form of the
actin machinery is essential to understanding a cell's functional interactions. To determine …

In this study, the mechanisms of actin-bundling in filopodia were examined. Analysis of
cellular localization of known actin cross-linking proteins in mouse melanoma B16F1 cells …

The morphogenesis of myosin II structures in active lamella undergoing net protrusion was
analyzed by correlative fluorescence and electron microscopy. In rat embryo fibroblasts (REF …