The distribution of neurons and terminal fields that contain a variety of neurotransmitters and steroid hormone receptors has been examined with in situ hybridization and immunohistochemistry in closely spaced series of sections throughout the rostrocaudal extent of the rat lateral septal nucleus, as well as the adjacent septohippocampal and septofimbrial nuclei. The results indicate that the lateral septal nucleus is divided into major rostral, caudal, and ventral parts that differ from the widely used cytoarchitectonic parcellation into dorsal, intermediate, and ventral parts. Furthermore, the rostral, caudal, and ventral parts are turn divided into about 20 zones, regions, and domains on the basis of differential terminal fields and neurons that express particular neuropeptides and steroid hormone receptors. In general, the small zones and regions form dorsoventrally oriented sheets or bands that are arranged in a complex way. Differential connections of these lateral septal components are analyzed in the accompanying paper (Risold, P. Y. and Swanson, L. W., Connections of the rat lateral septal complex, Brain Res. Rev., 24 (1997) 115-195).