Abstract
Using the phage P1-derived Cre/loxP recombination system, we have developed a method to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region. The Cre/loxP recombination-based gene deletion appears to require a certain level of Cre protein expression. The brain subregional restricted gene knockout should allow a more precise analysis of the impact of a gene mutation on animal behaviors.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Calcium-Calmodulin-Dependent Protein Kinases / genetics
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Gene Expression
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Gene Expression Regulation
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Genetic Engineering / methods*
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Gestational Age
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Hippocampus / physiology*
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Integrases / genetics*
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Mice
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Mice, Knockout / genetics*
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Mice, Transgenic
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Promoter Regions, Genetic
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RNA, Messenger / genetics
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Recombination, Genetic
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Sequence Deletion
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Viral Proteins*
Substances
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RNA, Messenger
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Viral Proteins
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Calcium-Calmodulin-Dependent Protein Kinases
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Cre recombinase
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Integrases