Abstract
Voltage-dependent sodium channels are involved in the initiation and propagation of action potentials in many excitable cells. Here we report that tipE, a gene defined by a temperature-sensitive paralytic mutation in Drosophila, encodes a novel integral membrane protein that dramatically stimulates functional expression in Xenopus oocytes of the Drosophila sodium channel alpha subunit encoded by the paralytic (para) locus. Using a heat shock promoter to control tipE+ gene expression in transgenic flies, we demonstrate that tipE+ gene expression is required during pupal development to rescue adult paralysis. In addition, we demonstrate a role for the tipE gene product in adults.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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Codon, Terminator / genetics
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DNA, Complementary / analysis
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DNA, Complementary / isolation & purification
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Drosophila
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Drosophila Proteins*
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Gene Expression Regulation, Developmental / physiology
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Genes, Insect / physiology
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Hot Temperature
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Membrane Proteins / genetics*
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Membrane Proteins / isolation & purification*
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Membrane Proteins / physiology*
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Molecular Sequence Data
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Mutation / genetics
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Oocytes / physiology
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Oocytes / ultrastructure
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Paralysis / genetics
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Pupa / genetics
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Pupa / ultrastructure
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Sodium Channels / genetics*
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Xenopus
Substances
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Codon, Terminator
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DNA, Complementary
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Drosophila Proteins
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Membrane Proteins
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Sodium Channels
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TipE protein, Drosophila