Plant-Derived Tick Repellents Activate the Honey Bee Ectoparasitic Mite TRPA1

Guangda Peng; Makiko Kashio; Tomomi Morimoto; Tianbang Li; Jingting Zhu; Makoto Tominaga; Tatsuhiko Kadowaki

doi:10.1016/j.celrep.2015.06.025

Plant-Derived Tick Repellents Activate the Honey Bee Ectoparasitic Mite TRPA1

Cell Rep. 2015 Jul 14;12(2):190-202. doi: 10.1016/j.celrep.2015.06.025. Epub 2015 Jul 2.

Authors

Guangda Peng¹, Makiko Kashio², Tomomi Morimoto³, Tianbang Li¹, Jingting Zhu¹, Makoto Tominaga⁴, Tatsuhiko Kadowaki⁵

Affiliations

¹ Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, 111 Ren'ai Road, Suzhou Dushu Lake Higher Education Town, Jiangsu Province 215123, China.
² Division of Cell Signaling, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki 444-8787, Japan.
³ Nagaragawa Research Center, API Co., Ltd., 692-3, Nagara, Gifu 502-0071, Japan.
⁴ Division of Cell Signaling, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki 444-8787, Japan; Department of Physiological Sciences, SOKENDAI (The Graduate University for Advanced Studies), Okazaki 444-8585, Japan.
⁵ Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, 111 Ren'ai Road, Suzhou Dushu Lake Higher Education Town, Jiangsu Province 215123, China. Electronic address: tatsuhiko.kadowaki@xjtlu.edu.cn.

PMID: 26146083
DOI: 10.1016/j.celrep.2015.06.025

Abstract

We have identified and characterized the TRPA1 channel of Varroa destructor (VdTRPA1), a major ectoparasitic mite of honey bee. One of the two VdTRPA1 isoforms, VdTRPA1L, was activated by a variety of plant-derived compounds, including electrophilic compounds, suggesting that chemical activation profiles are mostly shared between arthropod TRPA1 channels. Nevertheless, carvacrol and α-terpineol activated VdTRPA1L but not a honey bee noxious-stimuli-sensitive TRPA, AmHsTRPA, and Drosophila melanogaster TRPA1. Activation of VdTRPA1L in D. melanogaster taste neurons by the above compounds was sufficient to modify the gustatory behaviors. Carvacrol and α-terpineol repelled V. destructor in a laboratory assay, and α-terpineol repressed V. destructor entry for reproduction into the brood cells in hives. Understanding the functions of parasite TRP channels not only gives clues about the evolving molecular and cellular mechanisms of parasitism but also helps in the development of control methods.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Arthropod Proteins / chemistry
Arthropod Proteins / metabolism*
Bees / metabolism
Behavior, Animal / drug effects
Calcium / metabolism
Cyclohexane Monoterpenes
Cyclohexenes / chemistry
Cyclohexenes / isolation & purification
Cyclohexenes / pharmacology*
Cymenes
Drosophila melanogaster / metabolism
Gene Expression Regulation / drug effects*
HEK293 Cells
Humans
Insect Control
Molecular Sequence Data
Monoterpenes / chemistry
Monoterpenes / isolation & purification
Monoterpenes / pharmacology*
Plants / chemistry
Plants / metabolism
Protein Isoforms / chemistry
Protein Isoforms / genetics
Protein Isoforms / metabolism
Sequence Alignment
Transient Receptor Potential Channels / chemistry
Transient Receptor Potential Channels / genetics
Transient Receptor Potential Channels / metabolism*
Varroidae / metabolism*

Substances

Arthropod Proteins
Cyclohexane Monoterpenes
Cyclohexenes
Cymenes
Monoterpenes
Protein Isoforms
Transient Receptor Potential Channels
alpha-terpineol
carvacrol
Calcium

Associated data

GENBANK/AB930121
GENBANK/AB930122