Abstract
The sensory circumventricular organs (CVOs) comprise the organum vasculosum of the lamina terminalis (OVLT), subfornical organ (SFO), and area postrema (AP) and lack the blood-brain barrier. The expression of Toll-like receptor 4 (TLR4) was seen at astrocytes throughout the sensory CVOs and at microglia in the AP and solitary nucleus around the central canal. The peripheral and central administration of lipopolysaccharide induced a similar pattern of nuclear translocation of STAT3. A microglia inhibitor minocycline largely suppressed lipopolysaccharide-induced astrocytic nuclear translocation of STAT3 in the OVLT and AP, but its effect was less in the SFO.
Keywords:
Astrocyte; Brain; Fever; Inflammation; Microglia.
Copyright © 2014 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Astrocytes / drug effects
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Astrocytes / metabolism
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Brain / anatomy & histology*
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Brain / drug effects
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Calcium-Binding Proteins / metabolism
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Circumventricular Organs / drug effects*
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Dose-Response Relationship, Drug
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Drug Administration Routes
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Gene Expression Regulation / drug effects*
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Glial Fibrillary Acidic Protein / metabolism
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Laminin / metabolism
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Leukocyte Common Antigens / metabolism
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Lipopolysaccharides / pharmacology*
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Male
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Mice
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Mice, Inbred ICR
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Microfilament Proteins / metabolism
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Microscopy, Confocal
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Minocycline / pharmacology
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STAT3 Transcription Factor / metabolism*
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Time Factors
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Toll-Like Receptor 4 / metabolism*
Substances
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Aif1 protein, mouse
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Calcium-Binding Proteins
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Glial Fibrillary Acidic Protein
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Laminin
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Lipopolysaccharides
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Microfilament Proteins
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STAT3 Transcription Factor
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Tlr4 protein, rat
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Toll-Like Receptor 4
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Leukocyte Common Antigens
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Minocycline