Photothrombotic ischemia: a minimally invasive and reproducible photochemical cortical lesion model for mouse stroke studies

J Vis Exp. 2013 Jun 9:(76):50370. doi: 10.3791/50370.

Abstract

The photothrombotic stroke model aims to induce an ischemic damage within a given cortical area by means of photo-activation of a previously injected light-sensitive dye. Following illumination, the dye is activated and produces singlet oxygen that damages components of endothelial cell membranes, with subsequent platelet aggregation and thrombi formation, which eventually determines the interruption of local blood flow. This approach, initially proposed by Rosenblum and El-Sabban in 1977, was later improved by Watson in 1985 in rat brain and set the basis of the current model. Also, the increased availability of transgenic mouse lines further contributed to raise the interest on the photothrombosis model. Briefly, a photosensitive dye (Rose Bengal) is injected intraperitoneally and enters the blood stream. When illuminated by a cold light source, the dye becomes activated and induces endothelial damage with platelet activation and thrombosis, resulting in local blood flow interruption. The light source can be applied on the intact skull with no need of craniotomy, which allows targeting of any cortical area of interest in a reproducible and non-invasive way. The mouse is then sutured and allowed to wake up. The evaluation of ischemic damage can be quickly accomplished by triphenyl-tetrazolium chloride or cresyl violet staining. This technique produces infarction of small size and well-delimited boundaries, which is highly advantageous for precise cell characterization or functional studies. Furthermore, it is particularly suitable for studying cellular and molecular responses underlying brain plasticity in transgenic mice.

Publication types

  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

MeSH terms

  • Animals
  • Brain Ischemia / blood
  • Brain Ischemia / etiology*
  • Brain Ischemia / pathology
  • Disease Models, Animal*
  • Female
  • Intracranial Thrombosis / blood
  • Intracranial Thrombosis / etiology*
  • Intracranial Thrombosis / pathology
  • Mice
  • Mice, Transgenic
  • Neuronal Plasticity / physiology
  • Photochemical Processes
  • Rose Bengal / administration & dosage
  • Rose Bengal / chemistry
  • Stroke / blood
  • Stroke / etiology*
  • Stroke / pathology

Substances

  • Rose Bengal