High-resolution melting analysis (HRMA): a highly sensitive inexpensive genotyping alternative for population studies

B L Smith; C-P Lu; J R Alvarado Bremer

doi:10.1111/j.1755-0998.2009.02726.x

High-resolution melting analysis (HRMA): a highly sensitive inexpensive genotyping alternative for population studies

Mol Ecol Resour. 2010 Jan;10(1):193-6. doi: 10.1111/j.1755-0998.2009.02726.x. Epub 2009 Jun 15.

Authors

B L Smith¹, C-P Lu, J R Alvarado Bremer

Affiliation

¹ Department of Wildlife and Fisheries Sciences, Texas A&M University, 210 Nagle Hall, TAMU 2258, College Station, TX 77843, USA Department of Marine Biology, Texas A&M University at Galveston, 5007 Ave. U, Galveston, TX 77551, USA.

PMID: 21565009
DOI: 10.1111/j.1755-0998.2009.02726.x

Abstract

High-resolution melting analysis (HRMA) is a highly sensitive closed-tube genotyping method used primarily in clinical studies. As the method is rapid, inexpensive and amenable to high throughput, we decided to investigate its applicability to population studies. Small amplicons and unlabelled probes were used to genotype the nuclear genes, lactate dehydrogenase-A (ldh-A), myosin light chain-2 (mlc-2), acidic ribosomal phosphoprotein P0 (ARP) and calmodulin (CaM) in populations of swordfish, Xiphias gladius. Results indicate that HRMA is a powerful genotyping tool to study wild populations.