Abstract
Combining voltage and Ca(2+) imaging allows the correlation of electrical and chemical activity at sub-cellular level. Here we describe a novel apparatus designed to obtain simultaneous voltage and Ca(2+) measurements with single-trial resolution from sites as small as a few microns. These measurements can be obtained with negligible optical cross-talk between the two signals and negligible photo-damage of the preparation. The capability of the technique was assessed recording either from individual neurons in brain slices or from networks of cultured neurons. The present achievements open the gate to many novel physiological investigations requiring simultaneous measurement of voltage and Ca(2+) signals.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Action Potentials / drug effects
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Animals
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Calcium Signaling / drug effects
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Calcium Signaling / physiology*
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Cerebellum / cytology
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Cerebellum / physiology
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Electrophysiology / instrumentation
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Electrophysiology / methods
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Excitatory Postsynaptic Potentials / physiology
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Fluorescent Dyes / metabolism
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Fluorometry / instrumentation
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Fluorometry / methods*
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Fura-2 / analogs & derivatives
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Fura-2 / metabolism
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Hippocampus / cytology
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Hippocampus / physiology
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Membrane Potentials / drug effects
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Membrane Potentials / physiology*
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Mice
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Mice, Inbred C57BL
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Microscopy, Fluorescence / instrumentation
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Microscopy, Fluorescence / methods
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Picrotoxin / pharmacology
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Purkinje Cells / physiology
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Pyramidal Cells / drug effects
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Pyramidal Cells / physiology
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Pyridinium Compounds / metabolism
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Spectrometry, Fluorescence / instrumentation
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Spectrometry, Fluorescence / methods
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Styrenes / metabolism
Substances
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1-(3-sulfonatopropyl)-4-(beta-(2-(di-n-octylamino)-6-naphthyl)vinyl)pyridinium betaine
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Fluorescent Dyes
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JPW 1114
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Pyridinium Compounds
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Styrenes
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Picrotoxin
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Fura-2