Abstract
In cultured rat hippocampal neurons, we found that thrombospondin 1 (TSP1) increased the speed of synapse formation in young neurons, but not the final density of synapses in mature neurons. TSP1 interacted with neuroligin 1 (NL1) and application of the NL1 extracellular domain blocked TSP1-induced synaptogenesis. Furthermore, knocking down endogenous NL1 inhibited TSP1's effect. Our results indicate that TSP1 accelerates the speed of synaptogenesis through NL1 in hippocampal neurons.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / genetics
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Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / metabolism
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Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / pharmacology
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Cell Adhesion Molecules, Neuronal
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Cells, Cultured
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Disks Large Homolog 4 Protein
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Embryo, Mammalian
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Gene Expression Regulation / drug effects
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Gene Expression Regulation / physiology
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Green Fluorescent Proteins / genetics
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Guanylate Kinases
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Hippocampus / cytology*
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Humans
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Immunoprecipitation / methods
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Intracellular Signaling Peptides and Proteins / metabolism
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Luminescent Proteins / genetics
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Mice
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Mutagenesis, Site-Directed / methods
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism*
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Neurons / cytology*
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Protein Binding
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RNA, Small Interfering / pharmacology
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Rats
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Rats, Sprague-Dawley
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Receptors, LDL / genetics
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Receptors, LDL / metabolism
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Synapses / drug effects*
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Synapses / physiology
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Synapsins / metabolism
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Thrombospondin 1 / genetics
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Thrombospondin 1 / pharmacology*
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Transfection / methods
Substances
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Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
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Cell Adhesion Molecules, Neuronal
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Disks Large Homolog 4 Protein
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Dlg4 protein, mouse
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Intracellular Signaling Peptides and Proteins
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Luminescent Proteins
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Membrane Proteins
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Nerve Tissue Proteins
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RNA, Small Interfering
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Receptors, LDL
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SPZ1 protein, human
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Synapsins
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Thrombospondin 1
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VLDL receptor
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neuroligin 1
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Green Fluorescent Proteins
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Guanylate Kinases