Periventricular notch activation and asymmetric Ngn2 and Tbr2 expression in pair-generated neocortical daughter cells

Wataru Ochiai; Sayaka Nakatani; Taishi Takahara; Masahiko Kainuma; Makoto Masaoka; Sayaka Minobe; Masakazu Namihira; Kinichi Nakashima; Akira Sakakibara; Masaharu Ogawa; Takaki Miyata

doi:10.1016/j.mcn.2008.10.007

Periventricular notch activation and asymmetric Ngn2 and Tbr2 expression in pair-generated neocortical daughter cells

Mol Cell Neurosci. 2009 Feb;40(2):225-33. doi: 10.1016/j.mcn.2008.10.007. Epub 2008 Nov 7.

Authors

Wataru Ochiai¹, Sayaka Nakatani, Taishi Takahara, Masahiko Kainuma, Makoto Masaoka, Sayaka Minobe, Masakazu Namihira, Kinichi Nakashima, Akira Sakakibara, Masaharu Ogawa, Takaki Miyata

Affiliation

¹ Department of Anatomy and Cell Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa, Nagoya, Aichi 466-8550, Japan.

PMID: 19059340
DOI: 10.1016/j.mcn.2008.10.007

Abstract

To understand the cellular and molecular mechanisms regulating cytogenesis within the neocortical ventricular zone, we examined at high resolution the spatiotemporal expression patterns of Ngn2 and Tbr2. Individually DiI-labeled daughter cells were tracked from their birth in slice cultures and immunostained for Ngn2 and Tbr2. Both proteins were initially absent from daughter cells during the first 2 h. Ngn2 expression was then initiated asymmetrically in one of the daughter cells, with a bias towards the apical cell, followed by a similar pattern of expression for Tbr2, which we found to be a direct target of Ngn2. How this asymmetric Ngn2 expression is achieved is unclear, but gamma-secretase inhibition at the birth of daughter cells resulted in premature Ngn2 expression, suggesting that Notch signaling in nascent daughter cells suppresses a Ngn2-Tbr2 cascade. Many of the nascent cells exhibited pin-like morphology with a short ventricular process, suggesting periventricular presentation of Notch ligands to these cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Basic Helix-Loop-Helix Transcription Factors / genetics
Basic Helix-Loop-Helix Transcription Factors / metabolism*
Benzodiazepinones / pharmacology
Cyclin-Dependent Kinase Inhibitor p27 / genetics
Cyclin-Dependent Kinase Inhibitor p27 / metabolism
Gene Expression Regulation, Developmental*
Mice
Neocortex* / cytology
Neocortex* / embryology
Neocortex* / metabolism
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism*
Neurogenesis / physiology*
Promoter Regions, Genetic
Receptors, Notch / genetics
Receptors, Notch / metabolism
Signal Transduction / physiology
Stem Cells / cytology
Stem Cells / drug effects
Stem Cells / physiology*
T-Box Domain Proteins / genetics
T-Box Domain Proteins / metabolism*

Substances

2-(((3,5-difluorophenyl)acetyl)amino)-N-(1-methyl-2-oxo-5-phenyl-2,3-dihydro-1H-1,4-benzodiazepin-3-yl)propanamide
Basic Helix-Loop-Helix Transcription Factors
Benzodiazepinones
Eomes protein, mouse
Nerve Tissue Proteins
Neurog2 protein, mouse
Receptors, Notch
T-Box Domain Proteins
Cyclin-Dependent Kinase Inhibitor p27