Abstract
Platelet-derived growth factor alpha receptor (PDGFRA)/NG2-expressing glia are distributed throughout the adult CNS. They are descended from oligodendrocyte precursors (OLPs) in the perinatal CNS, but it is not clear whether they continue to generate myelinating oligodendrocytes or other differentiated cells during normal adult life. We followed the fates of adult OLPs in Pdgfra-creER(T2)/Rosa26-YFP double-transgenic mice and found that they generated many myelinating oligodendrocytes during adulthood; >20% of all oligodendrocytes in the adult mouse corpus callosum were generated after 7 weeks of age, raising questions about the function of the late-myelinating axons. OLPs also produced some myelinating cells in the cortex, but the majority of adult-born cortical cells did not appear to myelinate. We found no evidence for astrocyte production in gray or white matter. However, small numbers of projection neurons were generated in the forebrain, especially in the piriform cortex, which is the main target of the olfactory bulb.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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2',3'-Cyclic-Nucleotide Phosphodiesterases / metabolism
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Adult Stem Cells / drug effects
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Adult Stem Cells / physiology*
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Animals
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Antigens / metabolism*
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Basic Helix-Loop-Helix Transcription Factors / metabolism
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Bromodeoxyuridine / metabolism
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Cell Differentiation / drug effects
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Cell Differentiation / physiology
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Cerebral Cortex / cytology*
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Cerebral Cortex / physiology
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Estrogen Antagonists / pharmacology
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Gene Expression Regulation / drug effects
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Gene Expression Regulation / genetics
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Humans
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Luminescent Proteins / biosynthesis
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Luminescent Proteins / genetics
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Mice
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Mice, Transgenic
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Myelin Basic Protein / metabolism
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Nerve Tissue Proteins / metabolism
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Neural Pathways / physiology
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Neurons / physiology*
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Oligodendrocyte Transcription Factor 2
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Oligodendroglia / physiology*
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Phosphopyruvate Hydratase / metabolism
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Proteins / genetics
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Proteins / metabolism
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Proteoglycans / metabolism*
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RNA, Untranslated
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Receptor, Fibroblast Growth Factor, Type 3 / genetics
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Receptor, Platelet-Derived Growth Factor alpha / genetics
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Receptor, Platelet-Derived Growth Factor alpha / physiology*
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SOXE Transcription Factors / metabolism
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Tamoxifen / pharmacology
Substances
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Antigens
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Basic Helix-Loop-Helix Transcription Factors
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Estrogen Antagonists
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Gt(ROSA)26Sor non-coding RNA, mouse
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Luminescent Proteins
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Myelin Basic Protein
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Nerve Tissue Proteins
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Olig2 protein, mouse
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Oligodendrocyte Transcription Factor 2
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Proteins
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Proteoglycans
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RNA, Untranslated
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SOXE Transcription Factors
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Sox10 protein, mouse
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chondroitin sulfate proteoglycan 4
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Tamoxifen
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Receptor, Fibroblast Growth Factor, Type 3
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Receptor, Platelet-Derived Growth Factor alpha
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2',3'-Cyclic-Nucleotide Phosphodiesterases
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Phosphopyruvate Hydratase
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Bromodeoxyuridine