Abstract
Pro- and mature BDNF activate very different receptors and intracellular pathways, potentially leading to either neuronal death or survival. Here we examined the biochemistry of endogenous BDNF in mouse neurons using sensitive reagents and found that pro-BDNF is rapidly converted intracellularly to mature BDNF, the latter being stored and released by excitatory input.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Brain-Derived Neurotrophic Factor / deficiency
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Brain-Derived Neurotrophic Factor / metabolism*
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COS Cells
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Chlorocebus aethiops
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Dose-Response Relationship, Radiation
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Electric Stimulation / methods
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Enzyme-Linked Immunosorbent Assay
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Hippocampus / cytology*
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Immunoprecipitation / methods
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Long-Term Potentiation / physiology
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Long-Term Potentiation / radiation effects
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Methionine / metabolism
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Mice
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Mice, Knockout
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Neurons / metabolism*
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Patch-Clamp Techniques
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Protein Precursors / metabolism*
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Synaptic Transmission / physiology
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Synaptic Transmission / radiation effects
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Transfection / methods
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Tritium / metabolism
Substances
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Brain-Derived Neurotrophic Factor
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Protein Precursors
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brain-derived neurotrophic factor precursor
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Tritium
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Methionine