Abstract
Morphine modulates neuronal and immune cell functions via mu-opioid receptors. In primary and Jurkat T cells, and Raji B cells mu-opioid receptor transcripts were detected only after stimulation of the cells with IL-4 or TNF-alpha. Moreover, the amount of the induced mu-opioid receptor mRNA in the immune cells was 15 to 200 times less than those in primary cortical and SH SY5Y neuronal cells. Nevertheless, mu-opioid receptor mRNA in immune cells is processed to functional receptors, as demonstrated by morphine-mediated phosphorylation of mitogen activated protein kinase, morphine-mediated up-regulation of IL-4 mRNA and coupling to adenylyl cyclase in Jurkat cells.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cells, Cultured
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Cerebral Cortex / cytology
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Cyclic AMP / metabolism
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Embryo, Mammalian
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Gene Expression Regulation / immunology
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Gene Expression Regulation / physiology*
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Glutamate Decarboxylase / genetics
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Glutamate Decarboxylase / metabolism
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Humans
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In Situ Hybridization
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Interleukin-4 / pharmacology
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Mitogen-Activated Protein Kinases / genetics
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Mitogen-Activated Protein Kinases / metabolism
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Morphine / pharmacology
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Narcotics / pharmacology
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Neuroblastoma
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Neurons / metabolism*
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Rats
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Receptors, Opioid, mu / genetics
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Receptors, Opioid, mu / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction
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Somatostatin / analogs & derivatives
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Somatostatin / pharmacology
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T-Lymphocytes / drug effects
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T-Lymphocytes / metabolism*
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Tumor Necrosis Factor-alpha / pharmacology
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Vesicular Glutamate Transport Protein 1 / genetics
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Vesicular Glutamate Transport Protein 1 / metabolism
Substances
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Narcotics
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Receptors, Opioid, mu
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Tumor Necrosis Factor-alpha
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Vesicular Glutamate Transport Protein 1
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phenylalanyl-cyclo(cysteinyltyrosyl-tryptophyl-ornithyl-threonyl-penicillamine)threoninamide
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Interleukin-4
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Somatostatin
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Morphine
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Cyclic AMP
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Mitogen-Activated Protein Kinases
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Glutamate Decarboxylase