Comparison between basal and apical dendritic spines in estrogen-induced rapid spinogenesis of CA1 principal neurons in the adult hippocampus

Gen Murakami; Tomokazu Tsurugizawa; Yusuke Hatanaka; Yoshimasa Komatsuzaki; Nobuaki Tanabe; Hideo Mukai; Yasushi Hojo; Shiro Kominami; Takeshi Yamazaki; Tetsuya Kimoto; Suguru Kawato

doi:10.1016/j.bbrc.2006.10.066

Comparison between basal and apical dendritic spines in estrogen-induced rapid spinogenesis of CA1 principal neurons in the adult hippocampus

Biochem Biophys Res Commun. 2006 Dec 15;351(2):553-8. doi: 10.1016/j.bbrc.2006.10.066. Epub 2006 Oct 19.

Authors

Gen Murakami¹, Tomokazu Tsurugizawa, Yusuke Hatanaka, Yoshimasa Komatsuzaki, Nobuaki Tanabe, Hideo Mukai, Yasushi Hojo, Shiro Kominami, Takeshi Yamazaki, Tetsuya Kimoto, Suguru Kawato

Affiliation

¹ Department of Biophysics and Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3-8-1, Meguro, Tokyo 153, Japan.

PMID: 17070772
DOI: 10.1016/j.bbrc.2006.10.066

Abstract

Modulation of hippocampal synaptic plasticity by estrogen has been attracting much attention. Here, we demonstrated the rapid effect of 17beta-estradiol on the density and morphology of spines in the stratum oriens (s.o., basal side) and in the stratum lacunosum-moleculare (s.l.m., apical side) by imaging Lucifer Yellow-injected CA1 neurons in adult male rat hippocampal slices, because spines in s.o. and s.l.m. have been poorly understood as compared with spines in the stratum radiatum. The application of 1nM estradiol-induced a rapid increase in the density of spines of pyramidal neurons within 2h. This increase by estradiol was blocked by Erk MAP kinase inhibitor and estrogen receptor inhibitor in both regions. Effect of blockade by agonists of AMPA receptors and NMDA receptors was different between s.o. and s.l.m. In both regions, ERalpha agonist PPT induced the same enhancing effect of spinogenesis as that induced by estradiol.

Publication types

Comparative Study

MeSH terms

6-Cyano-7-nitroquinoxaline-2,3-dione / pharmacology
Animals
Dendritic Spines / drug effects
Dendritic Spines / physiology*
Estradiol / pharmacology*
Estrogen Receptor alpha / agonists
Estrogen Receptor alpha / antagonists & inhibitors
Estrogen Receptor alpha / metabolism
Extracellular Signal-Regulated MAP Kinases / antagonists & inhibitors
Extracellular Signal-Regulated MAP Kinases / metabolism
Flavonoids / pharmacology
Hippocampus / cytology
Hippocampus / drug effects
Hippocampus / physiology*
In Vitro Techniques
Male
Neuronal Plasticity
Neurons / cytology
Neurons / drug effects
Neurons / physiology*
Nitriles / pharmacology
Phenols
Pyramidal Cells / cytology
Pyramidal Cells / drug effects
Pyramidal Cells / physiology
Pyrazoles / pharmacology
Rats
Rats, Wistar
Receptors, AMPA / agonists
Receptors, AMPA / metabolism
Receptors, N-Methyl-D-Aspartate / agonists
Receptors, N-Methyl-D-Aspartate / metabolism

Substances

2,3-bis(4-hydroxyphenyl)-propionitrile
Estrogen Receptor alpha
Flavonoids
Nitriles
Phenols
Pyrazoles
Receptors, AMPA
Receptors, N-Methyl-D-Aspartate
4,4',4''-(4-propyl-((1)H)-pyrazole-1,3,5-triyl) tris-phenol
Estradiol
6-Cyano-7-nitroquinoxaline-2,3-dione
Extracellular Signal-Regulated MAP Kinases
2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one