Abstract
Multipotential bone marrow stromal cells (MSCs) from wild-type (Wt) or apolipoprotein E deficient (Apoe(-/-)) mice were implanted into the cerebral ventricles of Apoe(-/-) mice. MSCs from Wt mice continued expressing apoE up to 6 months after implantation and were associated with enhanced novel object recognition and increased microtubule-associated protein 2 (MAP2) immunoreactivity in the dentate gyrus. These data show that MSCs can be used to distinguish developmental from post-developmental effects of a gene knockout and support their therapeutic potential for neurodegenerative diseases.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Newborn
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Apolipoproteins E / genetics
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Apolipoproteins E / metabolism*
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Bone Marrow Cells / cytology
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Bone Marrow Cells / metabolism
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Bone Marrow Transplantation / methods
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Cerebral Ventricles / metabolism
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Cerebral Ventricles / surgery
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Dentate Gyrus / metabolism
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Female
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Immunohistochemistry
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Microtubule-Associated Proteins / metabolism
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Stromal Cells / cytology
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Stromal Cells / metabolism
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Stromal Cells / transplantation*
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Totipotent Stem Cells / cytology
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Totipotent Stem Cells / metabolism
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Totipotent Stem Cells / transplantation*
Substances
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Apolipoproteins E
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Microtubule-Associated Proteins
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Mtap2 protein, mouse