Abstract
Cannabinoids are powerful modulators of inhibition, yet the precise spike timing of cannabinoid receptor (CB1R)-expressing inhibitory neurons in relation to other neurons in the circuit is poorly understood. Here we found that the spike timing of CB1R-expressing basket cells, a major target for cannabinoids in the rat hippocampus, was distinct from the other main group of basket cells, the CB1R-negative. Despite receiving the same afferent inputs, the synaptic and biophysical properties of the two cell types were tuned to detect different features of activity. CB1R-negative basket cells responded reliably and immediately to subtle and repetitive excitation. In contrast, CB1R-positive basket cells responded later and did not follow repetitive activity, but were better suited to integrate the consecutive excitation of independent afferents. This temporal separation in the activity of the two basket cell types generated distinct epochs of somatic inhibition that were differentially affected by endocannabinoids.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Cannabinoids / metabolism*
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Cannabinoids / pharmacology
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Cell Shape / physiology
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Dendrites / drug effects
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Dendrites / metabolism
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Excitatory Postsynaptic Potentials / drug effects
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Excitatory Postsynaptic Potentials / physiology
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Hippocampus / cytology
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Hippocampus / drug effects
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Hippocampus / metabolism*
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Interneurons / drug effects
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Interneurons / metabolism*
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Male
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Nerve Net / cytology
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Nerve Net / drug effects
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Nerve Net / metabolism
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Neural Inhibition / drug effects
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Neural Inhibition / physiology*
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Neural Pathways / cytology
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Neural Pathways / drug effects
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Neural Pathways / metabolism
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Organ Culture Techniques
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Patch-Clamp Techniques
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Pyramidal Cells / drug effects
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Pyramidal Cells / physiology
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Rats
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Rats, Wistar
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Reaction Time / drug effects
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Reaction Time / physiology
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Receptor, Cannabinoid, CB1 / agonists
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Receptor, Cannabinoid, CB1 / antagonists & inhibitors
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Receptor, Cannabinoid, CB1 / metabolism*
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Synapses / drug effects
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Synapses / metabolism
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Synaptic Transmission / drug effects
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Synaptic Transmission / physiology*
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Time Factors
Substances
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Cannabinoids
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Receptor, Cannabinoid, CB1