Drosophila GPCR Han is a receptor for the circadian clock neuropeptide PDF

Seogang Hyun; Youngseok Lee; Sung-Tae Hong; Sunhoe Bang; Donggi Paik; Jongkyun Kang; Jinwhan Shin; Jaejung Lee; Keunhye Jeon; Seungyoon Hwang; Eunkyung Bae; Jaeseob Kim

doi:10.1016/j.neuron.2005.08.025

Drosophila GPCR Han is a receptor for the circadian clock neuropeptide PDF

Neuron. 2005 Oct 20;48(2):267-78. doi: 10.1016/j.neuron.2005.08.025.

Authors

Seogang Hyun¹, Youngseok Lee, Sung-Tae Hong, Sunhoe Bang, Donggi Paik, Jongkyun Kang, Jinwhan Shin, Jaejung Lee, Keunhye Jeon, Seungyoon Hwang, Eunkyung Bae, Jaeseob Kim

Affiliation

¹ Department of Biological Sciences, Korea Advanced Institute of Science & Technology, 373-1 Guseong-Dong, Yuseong-Gu, Daejeon 305-701, Korea.

PMID: 16242407
DOI: 10.1016/j.neuron.2005.08.025

Abstract

The pigment-dispersing factor (PDF) is a neuropeptide controlling circadian behavioral rhythms in Drosophila, but its receptor is not yet known. From a large-scale temperature preference behavior screen in Drosophila, we isolated a P insertion mutant that preferred different temperatures during the day and night. This mutation, which we named han, reduced the transcript level of CG13758. We found that Han was expressed specifically in 13 pairs of circadian clock neurons in the adult brain. han null flies showed arrhythmic circadian behavior in constant darkness. The behavioral characteristics of han null mutants were similar to those of pdf null mutants. We also found that PDF binds specifically to S2 cells expressing Han, which results in the elevation of cAMP synthesis. Therefore, we herein propose that Han is a PDF receptor regulating circadian behavioral rhythm through coordination of activities of clock neurons.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Antibodies / metabolism
Behavior, Animal
Binding, Competitive
Blotting, Northern / methods
Brain / cytology
Cell Line
Circadian Rhythm / physiology*
Cyclic AMP / metabolism
Dose-Response Relationship, Drug
Drosophila
Drosophila Proteins / genetics
Drosophila Proteins / immunology
Drosophila Proteins / metabolism*
Drosophila Proteins / physiology
Gene Expression Regulation / drug effects
Gene Expression Regulation / physiology*
Immunohistochemistry / methods
Motor Activity / physiology
Mutation
Neurons / metabolism
Neuropeptides / metabolism*
Periodicity
Protein Binding / physiology
RNA, Messenger / biosynthesis
Receptors, G-Protein-Coupled / genetics
Receptors, G-Protein-Coupled / immunology
Receptors, G-Protein-Coupled / metabolism*
Receptors, G-Protein-Coupled / physiology
Reverse Transcriptase Polymerase Chain Reaction / methods
Sequence Alignment
Thermosensing / genetics
Thermosensing / physiology

Substances

Antibodies
Drosophila Proteins
Neuropeptides
PDFR protein, Drosophila
RNA, Messenger
Receptors, G-Protein-Coupled
pdf protein, Drosophila
Cyclic AMP