A gene expression atlas of the central nervous system based on bacterial artificial chromosomes

Shiaoching Gong; Chen Zheng; Martin L Doughty; Kasia Losos; Nicholas Didkovsky; Uta B Schambra; Norma J Nowak; Alexandra Joyner; Gabrielle Leblanc; Mary E Hatten; Nathaniel Heintz

doi:10.1038/nature02033

A gene expression atlas of the central nervous system based on bacterial artificial chromosomes

Nature. 2003 Oct 30;425(6961):917-25. doi: 10.1038/nature02033.

Authors

Shiaoching Gong¹, Chen Zheng, Martin L Doughty, Kasia Losos, Nicholas Didkovsky, Uta B Schambra, Norma J Nowak, Alexandra Joyner, Gabrielle Leblanc, Mary E Hatten, Nathaniel Heintz

Affiliation

¹ GENSAT Project, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, Box 260, New York 10021, USA.

PMID: 14586460
DOI: 10.1038/nature02033

Abstract

The mammalian central nervous system (CNS) contains a remarkable array of neural cells, each with a complex pattern of connections that together generate perceptions and higher brain functions. Here we describe a large-scale screen to create an atlas of CNS gene expression at the cellular level, and to provide a library of verified bacterial artificial chromosome (BAC) vectors and transgenic mouse lines that offer experimental access to CNS regions, cell classes and pathways. We illustrate the use of this atlas to derive novel insights into gene function in neural cells, and into principal steps of CNS development. The atlas, library of BAC vectors and BAC transgenic mice generated in this screen provide a rich resource that allows a broad array of investigations not previously available to the neuroscience community.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Axons / metabolism
Cell Differentiation
Cell Lineage
Cell Movement
Central Nervous System / cytology
Central Nervous System / metabolism*
Chromosomes, Artificial, Bacterial / genetics*
Cyclic Nucleotide Phosphodiesterases, Type 1
Gene Expression Profiling* / methods
Gene Expression Regulation, Developmental
Gene Library*
Genes, Reporter / genetics*
Genetic Vectors / genetics
Green Fluorescent Proteins
Homeodomain Proteins / genetics
Homeodomain Proteins / metabolism
LIM-Homeodomain Proteins
Luminescent Proteins / analysis
Luminescent Proteins / genetics
Membrane Glycoproteins / genetics
Membrane Glycoproteins / metabolism
Mice
Mice, Transgenic
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism
Neurosciences / methods
Phosphoric Diester Hydrolases / genetics
Phosphoric Diester Hydrolases / metabolism
Synapses / metabolism
Transcription Factors
Transgenes / genetics*

Substances

Homeodomain Proteins
LHX6 protein, mouse
LIM-Homeodomain Proteins
Luminescent Proteins
Membrane Glycoproteins
Nerve Tissue Proteins
Transcription Factors
Green Fluorescent Proteins
Phosphoric Diester Hydrolases
Cyclic Nucleotide Phosphodiesterases, Type 1