Abstract
Using a subtraction cloning approach, we have isolated a set of cDNA clones from mouse neural precursor cells whose respective mRNA levels are down-regulated during the development of mouse brain. Single stranded DNA prepared from neuronal precursor cell cDNA library in lambda Zap vector was subtracted with poly (A)+ RNA prepared from postnatal and adult mouse brain to obtain several clones which show developmental down-regulation of expression. Their patterns of expression indicate that these genes may play important roles during the embryonic development and differentiation of central nervous system.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aging
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Animals
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Base Sequence
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Blotting, Northern
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Brain / embryology*
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Brain / growth & development*
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Cloning, Molecular
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DNA / genetics
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DNA / isolation & purification
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Embryo, Mammalian
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Embryonic and Fetal Development
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Gene Expression Regulation*
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Gene Library
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Genes*
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Gestational Age
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Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
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Mice
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Poly A / genetics
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Poly A / isolation & purification
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RNA / genetics
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RNA / isolation & purification
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
Substances
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Oligodeoxyribonucleotides
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RNA, Messenger
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Poly A
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RNA
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DNA
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Glyceraldehyde-3-Phosphate Dehydrogenases
Associated data
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GENBANK/D10712
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GENBANK/D10713
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GENBANK/D10714
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GENBANK/D10715
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GENBANK/D10915
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GENBANK/D10916
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GENBANK/D10917
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GENBANK/D10918
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GENBANK/D10919
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GENBANK/D10920