Abstract
Drosophila sensory neurons form distinctive terminal branch patterns in the developing neuropile of the embryonic central nervous system. In this paper we make a genetic analysis of factors regulating arbor position. We show that mediolateral position is determined in a binary fashion by expression (chordotonal neurons) or nonexpression (multidendritic neurons) of the Robo3 receptor for the midline repellent Slit. Robo3 expression is one of a suite of chordotonal neuron properties that depend on expression of the proneural gene atonal. Different features of terminal branches are separately regulated: an arbor can be shifted mediolaterally without affecting its dorsoventral location, and the distinctive remodeling of one arbor continues as normal despite this arbor shifting to an abnormal position in the neuropile.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Axons / metabolism
-
Axons / ultrastructure*
-
Basic Helix-Loop-Helix Transcription Factors
-
Cell Communication / genetics
-
Cell Differentiation / genetics*
-
DNA-Binding Proteins / genetics
-
DNA-Binding Proteins / metabolism*
-
Drosophila Proteins*
-
Drosophila melanogaster / cytology
-
Drosophila melanogaster / embryology*
-
Drosophila melanogaster / growth & development
-
Embryo, Nonmammalian / cytology
-
Embryo, Nonmammalian / embryology
-
Embryo, Nonmammalian / metabolism
-
Female
-
Functional Laterality / genetics
-
Gene Expression Regulation, Developmental / genetics*
-
Growth Cones / metabolism
-
Growth Cones / ultrastructure
-
Larva / cytology
-
Larva / growth & development
-
Larva / metabolism
-
Male
-
Membrane Proteins / genetics
-
Membrane Proteins / metabolism
-
Mutation / physiology
-
Nerve Tissue Proteins / genetics
-
Nerve Tissue Proteins / metabolism
-
Nervous System / cytology
-
Nervous System / embryology*
-
Nervous System / growth & development
-
Nervous System Malformations / genetics
-
Neuronal Plasticity / genetics
-
Neurons, Afferent / cytology
-
Neurons, Afferent / metabolism
-
Presynaptic Terminals / metabolism
-
Presynaptic Terminals / ultrastructure
-
Receptors, Immunologic / genetics
-
Receptors, Immunologic / metabolism*
Substances
-
Basic Helix-Loop-Helix Transcription Factors
-
DNA-Binding Proteins
-
Drosophila Proteins
-
Membrane Proteins
-
Nerve Tissue Proteins
-
Receptors, Immunologic
-
ato protein, Drosophila
-
comm protein, Drosophila
-
robo3 protein, Drosophila
-
sli protein, Drosophila