Abstract
Glucocorticoid receptor expression in primary hippocampal cell cultures was significantly increased with either 10 mM 8-bromo cAMP, 50 nM 5-carboxamidotryptamine (5-CT), a potent 5-HT7 receptor agonist, or 100 nM 5-HT. The effect of 5-HT or 5-CT was blocked with methiothepin or by a protein kinase A inhibitor, but not pindolol. These results suggest that the effects of 5-HT on hippocampal GR expression is mediated by a 5-HT7 receptor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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8-Bromo Cyclic Adenosine Monophosphate / pharmacology
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Animals
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Cells, Cultured
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Corticotropin-Releasing Hormone / metabolism*
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Cyclic AMP / analogs & derivatives
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Cyclic AMP / pharmacology
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Enzyme Inhibitors / pharmacology
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Feedback / physiology
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Fetus
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Habituation, Psychophysiologic / physiology
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Handling, Psychological
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Hippocampus / cytology
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Hippocampus / drug effects
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Hippocampus / metabolism*
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Hypothalamo-Hypophyseal System / metabolism*
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Hypothalamo-Hypophyseal System / physiopathology
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Rats
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Rats, Long-Evans
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Receptors, Glucocorticoid / drug effects
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Receptors, Glucocorticoid / metabolism*
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Receptors, Serotonin / drug effects
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Receptors, Serotonin / metabolism*
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Serotonin / metabolism*
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Serotonin / pharmacology
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Serotonin Antagonists / pharmacology
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Serotonin Receptor Agonists / pharmacology
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Stress, Physiological / metabolism*
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Stress, Physiological / physiopathology
Substances
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Enzyme Inhibitors
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Receptors, Glucocorticoid
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Receptors, Serotonin
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Serotonin Antagonists
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Serotonin Receptor Agonists
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serotonin 7 receptor
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8-Bromo Cyclic Adenosine Monophosphate
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Serotonin
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Corticotropin-Releasing Hormone
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Cyclic AMP