Abstract
Four new myoinhibitory peptides were isolated and identified from the ventral nerve cord of adult Manduca sexta. The new peptides are related to two previously identified myoinhibitory peptides also isolated from adult M. sexta, Mas-MIP I and Mas-MIP II. The sequences of the new peptides are APEKWAAFHGSWamide (Mas-MIP III), GWNDMSSAWamide (Mas-MIP IV), GWQDMSSAWamide (Mas-MIP V), and AWSALHGAWamide (Mas-MIP VI). Mas-MIPs III-VI were found to inhibit spontaneous peristalsis of the adult M. sexta anterior hindgut (ileum) in vitro.
MeSH terms
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Animals
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Chemical Fractionation
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Digestive System / drug effects
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Electrophysiology
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Insect Proteins / chemical synthesis
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Insect Proteins / isolation & purification
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Insect Proteins / physiology*
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Male
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Manduca
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Muscle Contraction / drug effects*
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Muscle Contraction / physiology
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Muscle, Smooth / drug effects
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Muscle, Smooth / physiology
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Neuromuscular Blocking Agents / chemical synthesis
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Neuromuscular Blocking Agents / isolation & purification
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Neuromuscular Blocking Agents / pharmacology*
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Neuropeptides / chemical synthesis
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Neuropeptides / isolation & purification
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Neuropeptides / physiology*
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Peripheral Nerves / chemistry
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Tissue Extracts / chemistry
Substances
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Insect Proteins
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Mas-MIP III protein, Manduca sexta
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Mas-MIP IV protein, Manduca sexta
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Mas-MIP V protein, Manduca sexta
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Mas-MIP VI protein, Manduca sexta
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Neuromuscular Blocking Agents
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Neuropeptides
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Tissue Extracts