Abstract
The combination of gene-targeting techniques in mouse embryonic stem cells and the Cre/loxP site-specific recombination system has resulted in the emergence of chromosomal-engineering technology in mice. This advance has opened up new opportunities for modelling human diseases that are associated with chromosomal rearrangements. It has also led to the generation of visibly marked deletions and balancer chromosomes in mice, which provide essential reagents for maximizing the efficiency of large-scale mutagenesis efforts and which will accelerate the functional annotation of mammalian genomes, including the human genome.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
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Review
MeSH terms
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Animals
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Chromosome Aberrations
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Chromosome Inversion
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Chromosomes / genetics*
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Chromosomes / ultrastructure
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Embryo, Mammalian / cytology
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Gene Duplication
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Gene Targeting / methods*
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Genetic Engineering / methods*
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Genetic Vectors / genetics
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Humans
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Hypoxanthine Phosphoribosyltransferase / genetics
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In Situ Hybridization, Fluorescence
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Integrases / physiology
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Mice / genetics*
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Mice, Transgenic
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Mutagenesis, Insertional
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RNA Splicing
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RNA, Messenger / genetics
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Recombination, Genetic
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Selection, Genetic
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Sequence Deletion
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Stem Cells
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Translocation, Genetic
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Viral Proteins / physiology
Substances
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RNA, Messenger
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Viral Proteins
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Hypoxanthine Phosphoribosyltransferase
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Cre recombinase
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Integrases