Abstract
Optical recording of neuronal activities using voltage-sensitive dyes (VSDs) is a useful method for simultaneous multi-site recording. However, it has been rather difficult to distinguish optical signals from individual, identified cells. We applied the optical recording technique using a high-speed charge coupled device (CCD) imaging system to a teleost thalamic nucleus, corpus glomerulosum (CG) which has a well-defined histological organization and large postsynaptic dendrites. Patch-like dye (di-4-ANEPPS) signals were observed in the dendritic layer of the CG in response to afferent nerve stimulations. These responses were completely blocked by an alpha-amino-3-hydroxy-5-methyl-4-isoxazole-proprionate (AMPA) receptor antagonist, did not propagate, and the size of the patches were close to that of a single dendritic tip of the 'large cell'. Thus, we found that these patch-like VSD signals most likely represent postsynaptic potentials at individual dendritic tips of the large cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Dendrites / drug effects
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Dendrites / physiology*
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Dendrites / ultrastructure
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Electronic Data Processing / instrumentation
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Electronic Data Processing / methods
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Excitatory Amino Acid Antagonists / pharmacology
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Fishes / anatomy & histology
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Fishes / physiology*
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Fluorescent Dyes
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Image Processing, Computer-Assisted / instrumentation
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Image Processing, Computer-Assisted / methods
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Membrane Potentials / drug effects
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Membrane Potentials / physiology
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Pyridinium Compounds
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Quinoxalines / pharmacology
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Receptors, AMPA / antagonists & inhibitors
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Receptors, AMPA / metabolism
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Synaptic Membranes / drug effects
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Synaptic Membranes / physiology*
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Synaptic Membranes / ultrastructure
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Thalamus / cytology
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Thalamus / drug effects
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Thalamus / physiology*
Substances
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Excitatory Amino Acid Antagonists
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Fluorescent Dyes
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Pyridinium Compounds
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Quinoxalines
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Receptors, AMPA
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2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline
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1-(3-sulfonatopropyl)-4-(beta)(2-(di-n-butylamino)-6-naphthylvinyl)pyridinium betaine