Abstract
The 5' flanking region of the alpha isoform of the rat Ca2+/calmodulin-dependent protein kinase II (alpha CaM kinase II) gene was isolated in 2.3 kbp of genomic sequence. Functional analysis of alpha CaM kinase II promoter deletion mutants fused to a reporter gene in neuroblastoma, including N18TG2, NG108-15, and CAD cells revealed strong transcriptional activity localized 100-145 bp, and a potent silencer 199-275 bp upstream of the transcription start site. The promoter is inactive in non-neuronal cells including BALB/c 3T3, Chinese hamster ovary, HT1080, and C6 glioma cells. These results indicated that the alpha CaM kinase II gene is transcribed from a tissue-specific promoter which is under intense negative control.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Base Sequence / physiology
-
Binding Sites / genetics
-
Brain / cytology
-
Brain / enzymology*
-
Brain Chemistry / genetics
-
Calcium-Calmodulin-Dependent Protein Kinase Type 2
-
Calcium-Calmodulin-Dependent Protein Kinases / genetics*
-
Gene Silencing / physiology
-
Genes / genetics*
-
Genes, Reporter / genetics
-
Humans
-
Luciferases / genetics
-
Mice
-
Molecular Sequence Data
-
Neurons / cytology
-
Neurons / enzymology*
-
Promoter Regions, Genetic / genetics*
-
Protein Isoforms / genetics
-
Transcription, Genetic / genetics*
-
Transfection
-
Tumor Cells, Cultured / metabolism*
Substances
-
Protein Isoforms
-
Luciferases
-
CAMK2A protein, human
-
Calcium-Calmodulin-Dependent Protein Kinase Type 2
-
Calcium-Calmodulin-Dependent Protein Kinases
-
Camk2a protein, mouse
-
Camk2a protein, rat