Abstract
Slit proteins are a family of secreted guidance proteins that can repel neuronal migration and axon growth via interaction with their cellular roundabout receptors (Robos). Here it was shown that Slit2-Robo-1 interactions were enhanced by cell-surface heparan sulfate. Removal of heparan sulfate decreased the affinity of Slit for Robo by about threefold. In addition, removal of cell-surface heparan sulfate by heparinase III abolished the chemorepulsive response to Slit2 normally shown by both the migrating neurons and growing axons. These results indicate essential roles for cell-surface heparan sulfate in the repulsive activities of Slit2.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Axons / physiology
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Cell Line
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Cell Movement
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Fluorescent Antibody Technique
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Heparan Sulfate Proteoglycans / metabolism
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Heparitin Sulfate / physiology*
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In Vitro Techniques
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Intercellular Signaling Peptides and Proteins
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Interneurons / physiology
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Mice
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Nerve Tissue Proteins / metabolism
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Nerve Tissue Proteins / physiology*
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Olfactory Bulb / cytology
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Olfactory Bulb / ultrastructure
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Rats
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Receptors, Immunologic / metabolism
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Roundabout Proteins
Substances
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Heparan Sulfate Proteoglycans
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Intercellular Signaling Peptides and Proteins
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Nerve Tissue Proteins
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Receptors, Immunologic
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Heparitin Sulfate
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Slit homolog 2 protein