Synapto-pHluorins: chimeras between pH-sensitive mutants of green fluorescent protein and synaptic vesicle membrane proteins as reporters of neurotransmitter release

Methods Enzymol. 2000:327:522-46. doi: 10.1016/s0076-6879(00)27300-x.

Authors

R Yuste¹, R B Miller, K Holthoff, S Zhang, G Miesenböck

Affiliation

¹ Department of Biological Sciences, Columbia University, New York, New York 10027, USA.

PMID: 11045007
DOI: 10.1016/s0076-6879(00)27300-x

No abstract available

MeSH terms

Animals
Brain / metabolism
Cell Line
Cell Membrane / metabolism*
Gene Transfer Techniques
Green Fluorescent Proteins
Herpesvirus 1, Human / metabolism
Hippocampus / cytology
Humans
Hydrogen-Ion Concentration
Luminescent Proteins / chemistry*
Luminescent Proteins / metabolism*
Microscopy, Fluorescence / instrumentation*
Microscopy, Fluorescence / methods*
Neurons / metabolism
Neurotransmitter Agents / metabolism*
Rats
Recombinant Fusion Proteins / chemistry*
Recombinant Fusion Proteins / metabolism*
Synapses / metabolism*

Substances

Luminescent Proteins
Neurotransmitter Agents
Recombinant Fusion Proteins
Green Fluorescent Proteins