Molecular characterisation of two structurally distinct groups of human homers, generated by extensive alternative splicing

J Mol Biol. 2000 Feb 4;295(5):1185-200. doi: 10.1006/jmbi.1999.3436.

Abstract

Homer proteins bind specifically to the C termini of the metabotropic glutamate receptor mGluR1alpha/a and mGluR5, play a role in their targeting and modulate their synaptic properties. We have discovered that extensive alternative splicing generates a family of 17 Homer proteins. These fall into two distinct groups of 12 "long" Homers, which all have a coiled-coil domain at their C termini, and five "short" Homers, which lack such a domain. All Homers contain the N-terminal sequence responsible for their binding to mGluR1alpha/a receptors and can be co-localised with the recombinantly expressed mGluR1alpha/a protein in HEK-293 cells. The existence of the long and the short variants of each of the Homer-1, Homer-2 and Homer-3 proteins reflects the fundamental principles of Homer functions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing / genetics*
  • Amino Acid Sequence
  • Base Sequence
  • Carrier Proteins / chemistry*
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cell Line
  • Cloning, Molecular
  • Fluorescent Antibody Technique
  • Gene Expression
  • Genetic Variation / genetics
  • HeLa Cells
  • Homer Scaffolding Proteins
  • Humans
  • Molecular Sequence Data
  • Molecular Weight
  • Neuropeptides / chemistry*
  • Neuropeptides / genetics*
  • Neuropeptides / metabolism
  • Protein Binding
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Receptors, Metabotropic Glutamate / genetics
  • Receptors, Metabotropic Glutamate / metabolism
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Structure-Activity Relationship
  • Transfection

Substances

  • Carrier Proteins
  • Homer Scaffolding Proteins
  • Neuropeptides
  • RNA, Messenger
  • Receptors, Metabotropic Glutamate
  • Recombinant Fusion Proteins
  • metabotropic glutamate receptor type 1

Associated data

  • GENBANK/AF081530
  • GENBANK/AF093264
  • GENBANK/AF093265
  • GENBANK/Y17572
  • GENBANK/Y17573