Abstract
Homer proteins bind specifically to the C termini of the metabotropic glutamate receptor mGluR1alpha/a and mGluR5, play a role in their targeting and modulate their synaptic properties. We have discovered that extensive alternative splicing generates a family of 17 Homer proteins. These fall into two distinct groups of 12 "long" Homers, which all have a coiled-coil domain at their C termini, and five "short" Homers, which lack such a domain. All Homers contain the N-terminal sequence responsible for their binding to mGluR1alpha/a receptors and can be co-localised with the recombinantly expressed mGluR1alpha/a protein in HEK-293 cells. The existence of the long and the short variants of each of the Homer-1, Homer-2 and Homer-3 proteins reflects the fundamental principles of Homer functions.
Copyright 2000 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing / genetics*
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Amino Acid Sequence
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Base Sequence
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Carrier Proteins / chemistry*
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism
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Cell Line
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Cloning, Molecular
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Fluorescent Antibody Technique
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Gene Expression
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Genetic Variation / genetics
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HeLa Cells
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Homer Scaffolding Proteins
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Humans
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Molecular Sequence Data
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Molecular Weight
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Neuropeptides / chemistry*
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Neuropeptides / genetics*
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Neuropeptides / metabolism
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Protein Binding
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Protein Structure, Secondary
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Protein Structure, Tertiary
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Receptors, Metabotropic Glutamate / genetics
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Receptors, Metabotropic Glutamate / metabolism
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Structure-Activity Relationship
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Transfection
Substances
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Carrier Proteins
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Homer Scaffolding Proteins
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Neuropeptides
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RNA, Messenger
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Receptors, Metabotropic Glutamate
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Recombinant Fusion Proteins
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metabotropic glutamate receptor type 1
Associated data
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GENBANK/AF081530
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GENBANK/AF093264
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GENBANK/AF093265
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GENBANK/Y17572
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GENBANK/Y17573