A simplified method of generating transgenic Xenopus

D B Sparrow; B Latinkic; T J Mohun

doi:10.1093/nar/28.4.e12

A simplified method of generating transgenic Xenopus

Nucleic Acids Res. 2000 Feb 15;28(4):E12. doi: 10.1093/nar/28.4.e12.

Authors

D B Sparrow¹, B Latinkic, T J Mohun

Affiliation

¹ Division of Developmental Biology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK. dsparro@nimr.mrc.ac.uk

Abstract

Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Animals, Genetically Modified*
Fertilization
Gene Transfer Techniques
Green Fluorescent Proteins
Luminescent Proteins / genetics
Male
Ovum
Sperm Injections, Intracytoplasmic
Spermatozoa
Xenopus / genetics*
Xenopus laevis

Substances

Luminescent Proteins
Green Fluorescent Proteins