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Journal / Biology / Preparation of Mouse Embryonic Fibroblast Cells…
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Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells
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Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells

Article DOI: 10.3791/3854-v • 09:38 min • June 21st, 2012
June 21st, 2012 •
1, 1, 1
1Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics

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Summary
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The quality of mouse embryonic fibroblasts (MEFs) is dictated by the right strain of mouse such as CF-1. Pluripotency-supportive MEFs and conditioned media (CM) obtained from these should contain optimal concentrations of Activin A, Gremlin and Tgfβ1 needed for the Activin/Nodal and FGF pathways to co-operatively maintain self-renewal and pluripotency.

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Mouse Embryonic Fibroblast Cells Culturing Human Embryonic Stem Cells Culturing Induced Pluripotent Stem Cells Culture Conditions Gene Expression Pluripotency Feeder Cells Fetal Bovine Serum Knockout Serum Replacement FGF2 Matrigel-coated Plates Conditioned Medium Chemically Defined Conditions Xeno-free Components Mouse Feeder Cells Human Cell Lines Fetal Muscle Cells Skin Cells Amniotic Mesenchymal Cells
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