Abstract
Models of acute nociception using a thermal stimulus are widely employed as screening methods for nociceptive properties of new drug compounds. In this chapter, detailed descriptions for conducting of two of the most commonly used models; the hot plate test and the “Hargreaves test,” are described. These models are applicable to both rats and mice and have the advantage of allowing repeated and multiple testing using a single animal because the stimulus is transitory and produces no tissue damage. Additionally, a modication of these models using a skin-twitch reflex that is applicable to large laboratory animals such a dogs or sheep is described. Guidance concerning potential confounding variable are discussed, as are tips for reducing variably among testing sessions.
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References
Treede, R. D., Meyer, R. A., Raja, S. N., and Campbell, J. N. (1992) Peripheral and central mechanisms of cutaneous hyperalgesia. Prog. Neurobiol. 38, 397–421.
Willis, W. D. and Westlund, K. N. (1997) Neuroanatomy of the pain system and of the pathways that modulate pain. J. Clin. Neurophysiol. 14, 2–31.
Tsuruoka, M., Matsui, A., and Matsui, Y. (1988) Quantitative relationship between the stimulus intensity and the response magnitude in the tail flick reflex. Physiol. Behav. 43, 79–83.
Dirig, D. M. and Yaksh, T. L. (1995) Differential right shifts in the dose-response curve for intrathecal morphine and sufentanil as a function of stimulus intensity. Pain 62, 321–328.
Le Bars, D., Gozariu, M., and Cadden, S. W. (2001) Animal models of nociception. Pharmacol. Rev. 53, 597–652.
Yaksh TL. Preclinical models of nociception, in Anesthesia: Biologic Foundations (Yaksh, T. L., Lynch III, C., Zapol, W. M., et al., eds.). Lippincott-Raven Publishers, Philadelphia, pp. 685–718.
D’Amour, F. E. and Smith, D. L. (1941) A method for determining loss of pain sensation. J. Phamacol. Exp. Ther. 72, 74–79.
Martin, W. R., Eades, C. G., Fraser, H. F., and Winkler, A. (1964) Use of hindlimb reflexes of the chronic spinal dog for comparing analgesics. J. Pharmacol. Exp. Ther. 144, 8–11.
Janssen, P. A. J., Niemegeers, C. J. E., and Dony, J. G. H. (1963) The inhibitory effect of fentanyl and other morphine like analgesics on the warm water induced tail withdrawal reflex in the rat. Arzneimittelforsch 13, 502–507.
Hargreaves, K., Dubner, R., Brown, F., Flores, C., and Joris, J. (1988) A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia. Pain 32, 77–88.
Sabbe, M. B., Grafe, M. R., Mjanger, E., et al. (1994) Spinal delivery of sufentanil, alfentanil, and morphine in dogs. Physiologic and toxicologic investigations. Anesthesiology 81, 899–920.
Yaksh, T. L. (ed.) (1999) Spinal Drug Delivery. Elsevier Science, New York, NY.
Defrin, R., Ohry, A., Blumen, N., and Urca, G. (2002) Sensory determinants of thermal pain. Brain 125, 501–510.
Dirig, D. M., Isakson, P. C., and Yaksh, T. L. (1998) Effect of COX-1 and COX-2 inhibition on induction and maintenance of carrageenan-evoked thermal hyperalgesia in rats. J. Pharmacol. Exp. Ther. 285, 1031–1037.
Hurley, R. W., Chatterjea, D., {mnRose Feng}, M., et al. (2002) Gabapentin and pregabalin can interact synergistically with naproxen to produce antihyperalgesia. Anesthesiology 97, 1263–1273.
Yaksh, T. L., Hua, X.-Y., Kalcheva, I., et al. (1999) The spinal biology in humans and animals of pain states generated by persistent small afferent input. Proc. Natl. Acad. Sci. USA 96, 7680–7686.
Yaksh, T. L. (1999) Spinal systems and pain processing, development of novel analgesic drugs with mechanistically defined models. Trends Pharmacol. Sci. 20, 329–337.
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Allen, J.W., Yaksh, T.L. (2004). Assessment of Acute Thermal Nociception in Laboratory Animals. In: Luo, Z.D. (eds) Pain Research. Methods in Molecular Medicine, vol 99. Humana Press. https://doi.org/10.1385/1-59259-770-X:139
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DOI: https://doi.org/10.1385/1-59259-770-X:139
Publisher Name: Humana Press
Print ISBN: 978-1-58829-103-5
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