Growth Retardation and Persistent Leanness in Tph2−/− but Age- and Sex-dependent Overweight in Tph2+/− Mice

5-HT is implicated in the regulation of various physiological pathways influencing somatic growth, appetite, energy expenditure and storage. To evaluate the effect of central 5-HT deficiency on the regulation of these mechanisms, body weight was determined in different Tph2 mutants compared to wildtype (wt) littermates at different ages from 3 weeks up to 2.2 years. First, as visible in Fig. 1, adult Tph2-deficient mice display an overall normal life expectancy. Using age as a covariable, growth retardation and leanness which persists throughout the lifespan was observed in Tph2−/− males (F_(2,417) = 11.56, p<0.001; Bonferroni-corrected pair-wise comparisons: −/− < wt and +/−, p<0.001) and Tph2−/− females (F_(2,370) = 14.624, p<0.001; −/− < wt, p = 0.02; −/− < +/−, p<0.001). During their first 24 weeks of life, Tph2−/− females had lower body weight than wt and +/− (F_(2,264) = 11.86, p<0.001, −/− < wt and +/−, p<0.001) but wt and Tph2+/− mice did not differ (p = 0.25). However, from 24 weeks of age onward, female +/− mice started to diverge from wt littermates showing an increase in their rate of weight gain (F_(2,88) = 15.95, p<0.001; wt < +/−, p = 0.031; −/− < wt, p = 0.013; −/− < +/−, p<0.001). Dissection revealed that Tph2+/− females can have impressive amount of fat stored in their abdominal and pericardial cavity, particularly in advanced age, while fat pads in Tph2−/− were much reduced compared to their littermates. Although Tph2+/− males also appeared to be more obese, their body weight did not significantly differ from wt controls before (F_(2,320) = 24.713, p<0.001; wt vs +/−, p = 1; −/− < wt, p<0.001; −/− < +/−, p<0.001) or after 24 weeks of age (F_(2,79) = 15.68, p<0.001; wt vs +/−, p = 0.46; −/− < wt, p<0.001; −/− < +/−, p<0.001). These results reveal a dual impact of central 5-HT in the regulation of somatic development and metabolic homeostasis and that brain 5-HT deficiency dose-dependently affects body weight via partially opposing mechanisms.

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Figure 1. Growth and body weight during lifespan.

Different male and female mice were weighted at different ages between 3 weeks and 2.2 years (421 males: wt, n = 155; +/−, n = 173; −/−, n = 93; and 374 females: wt, n = 147; +/−, n = 146; −/−, n = 81). Tph2−/− of both sexes displayed a growth retardation which persists throughout the lifespan as assessed by a constant significantly lower body weight than wt (males, p<0.001; females, p = 0.02) and +/− mice (males, p<0.001; females, p = 0.007). A significant sex-specific overweight was observed in Tph2+/− females from 24 weeks of age onward (+/− > wt, p = 0.031; +/− > −/−, p<0.001), while +/− males did not differ from wt. Age was used as covariable in the ANOVA analyses followed by Bonferroni-corrected pair-wise comparisons.

https://doi.org/10.1371/journal.pone.0043157.g001

Tph2 Inactivation Results in Brain 5-HT Deficiency and Reduction of Norepinephrine

To assess the effect of Tph2 inactivation on brain 5-HT and its influence on the function of other neurotransmitter systems, monoamine concentrations were first analyzed by high-performance liquid chromatography (HPLC) in different brain regions of phosphate buffered saline (PBS) perfused animals (Fig. 2A).

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Figure 2. Neurotransmitter concentrations and TH positive cells counting in different brain regions.

(A) HPLC analysis of norepinephrine (NE), dopamine (DA), serotonin (5-HT) and its metabolite 5-hydroxyindoleacetic acid (5-HIAA) in four different brain regions showed a drastic reduction of 5-HT and 5-HIAA concentrations in the brain of Tph2−/− compared to wt and +/− mice (n = 8). This reduction was further increased by an improved perfusion protocol (see corresponding results section) with e.g. 98.9% reduction in rostral raphe. A general reduction of NE concentration was also observed across brain regions of Tph2−/− mice compared to wt and +/− and a reduction of DA concentration in the hippocampus compared to wt mice. Kruskall-Wallis followed by Mann-Whitney-U-test: *p<0.05, **p<0.01, ***p<0.001. (B) Top: density of tyrosine hydroxylase (TH) positive cells in the locus coeruleus (LC, main noradrenergic cell cluster in the brain) showing a reduction of cell number in the anterior LC (aLC) of Tph2−/− mice (n = 8). Botom: density of TH positive cells in the main dopaminergic cell clusters: subtantia nigra (SN), ventral tegmental area (VTA) and retrorubal field A8 (n = 8). cLC: central LC, pLC: posterior LC. ANOVA followed by Tuckey-HSD: *p<0.05. Data are presented as means ± sem.

https://doi.org/10.1371/journal.pone.0043157.g002

Reduced Number of Noradrenergic Neurons in Tph2−/− Mice

To assess the effect of 5-HT deficiency on development and integrity of other neurotransmitter systems and to assess whether the reduction in NE and DA levels is due to a decreased number of catecholamine-specific neurons, noradrenergic and dopaminergic neurons were identified by tyrosine hydroxylase (TH) immunostaining and quantified in their respective nuclei (Fig. 2B). TH positive-cells were counted in the locus coeruleus (LC), the main central NE cell cluster, located in the brain stem as well as in the major dopaminergic nuclei, the substantia nigra (SN), ventral tegmental area (VTA) and A8 which, in its caudal boundary, is anatomically overlapping with the most rostral part of the dorsal raphe (DR). Counting of noradrenergic neurons in the LC revealed a significant reduction of cell densities in Tph2−/− mice in subparts of this cluster, rather than in the structure as a whole. Brain slices comprising anterior parts of LC exhibited a significant genotype effect (F_(2,17) = 5.23, p = 0.017) with a reduction of 33.8% (p = 0.015) in Tph2−/− and 24.9% (p = 0.081) in Tph2+/− mice compared to wt littermates. The difference between +/− and −/− groups was 11.9% without reaching significance (p = 0.668). In central LC, although Tph2−/− mice displayed a 17.5% reduction of cell density, the genotype effect was not significant (F_(2,23) = 1.63, p = 0.219). The posterior LC did not reveal a significant genotype effect (F_(2,19) = 2.43, p = 0.115). In the DA cell clusters SN, VTA and A8, no significant inter-genotypic difference was found. A detailed analysis within each cluster in a rostro-caudal dissection did also not show any difference. Since the volume of the cell clusters did not differ between genotypes in any of the analyzed structures, the cell density directly reflects the total number of TH-positive cells. The reduced NE brain concentration elicited by HPLC may then, at least in part, be explained by a reduction of the number of TH-positive noradrenergic neurons in the LC as a source of NE in terminal brain regions.

Serotonergic Molecular Phenotype of Raphe Neurons Devoid of 5-HT is Conserved

Given the known neurotrophic role of 5-HT in brain development, we have also investigated the effect of 5-HT synthesis incapacity on the development and differentiation of the serotonergic neurons themselves. Fig. 3a shows the absence of Tph2 immunoreactivity in Tph2−/− rostral raphe nuclei. It was also absent in other raphe nuclei and any further brain regions. We had previously shown the absence of 5-HT and Tph1 immunoreactivity in the brain of Tph2−/− mice ([5] and [7] respectively) by chromogenic immunohistochemistry. Here we additionally tested the other 5-HT specific markers. The 5-HT transporter (Sert) was normally present on the plasmatic soma membrane (Fig. 3b) as well as along projecting serotonergic fibers, e.g. in FC (Fig. 3c) but also in the other brain regions, such as Hip [5]. Immunofluorescent labeling confirmed the absence of specific 5-HT immunoreactivity in the raphe neurons (Fig. 3e) and other brain regions of Tph2−/− mice. The vesicular monoamine transporter-2 (Vmat2), which is in the raphe nuclei specifically expressed in wt 5-HT positive neurons (Fig. 3g), was also present in raphe neurons of Tph2−/− mice (Fig. 3f). Furthermore, using in situ hybridization, we demonstrated the maintained expression of the 5-HT neuron specific transcription factor Pet1 in the raphe of Tph2−/− mice (Fig. 3d). These results, together with 5-HT_1A and 5-HT_1B autoradiography in raphe (see below), demonstrate a serotonergic-like phenotype and apparently normal cellular and morphological differentiation of the neurons despite the absence of Tph2 and endogenous 5-HT synthesis.

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Figure 3. Histological characterization of serotonergic neurons.

Detection of serotonergic-specific markers was performed on coronal brain sections of adult wt control (left panel) and Tph2−/− mice (right panel). Protein labeling was obtained by light immunohistochemistry (a-c) and immunohistofluorescence (e-g). (a) Labeling of Tph2 demonstrated its complete absence in the raphe of Tph2−/− mice. (b) The serotonin transporter (Sert) could be detected in both wt and Tph2−/− mice, in the raphe as well as along fibers in projection areas, e.g. in the frontal cortex (FC) as shown in (c). (d) Detection of the serotonergic-specific transcription factor Pet1 in the raphe by in situ hybridization occurred similarly in wt and Tph2−/− mice. (e) Detection of serotonin (5-HT) in the raphe showed the absence of specific 5-HT immunoreactivity in Tph2−/− mice. Cell nuclei were also labeled by DAPI staining. (f) The vesicular monoamine transporter-2 (Vmat2) could be detected similarly in the raphe of both wt and Tph2−/− mice. (g) Merged images from (e-f) showed the colocalization of 5-HT and Vmat2 in the serotonergic neurons of wt (yellow in g) while Tph2−/− neurons were only labeled with Vmat2 (red in g). Taken together these results demonstrate that despite 5-HT synthesis deficiency, serotonergic neurons of Tph2−/− mice can develop and be maintained. Moreover, except Tph2 and 5-HT, they possess all known 5-HT-specific markers showing that their serotonergic specification took place. Bars represent 100 µm in (c) and 200 µm in (a), (b), (d), (e-g).

https://doi.org/10.1371/journal.pone.0043157.g003

Increased 5-HT_1A and 5-HT_1B Receptor Density and Stimulated [³⁵S]GTP-γ-S Binding

To evaluate the effect of 5-HT deficiency on the regulation of its autoreceptors at the pre- and post-synaptic level, we have quantified 5-HT_1A and 5-HT_1B receptors in relevant brain regions (Fig. 4).

Electrophysiological Properties of Serotonergic Neurons are Preserved in Tph2−/− Mice

Another critical feature of maturation and physiological function of a specific neuron population is the acquisition of its specific electrophysiological characteristics. To investigate whether Tph2 and thus 5-HT synthesis are required for the development and maintenance of 5-HT neuron-specific electrophysiological activity, we studied serotonergic DR neurons and recorded their spontaneous firing and response to various compounds in order to verify the complete absence of Tph activity and the functionality of autoinhibitory mechanisms (Fig. 5).

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Figure 4. Quantitative autoradiography of 5-HT_1A and 5-HT_1B receptors in various brain regions.

(A) Representative photomicrographs of autoradiograms following the binding of [³H]WAY100635 to 5-HT_1A receptors on whole coronal sections. The signal was visibly increased in e.g. the dorsal raphe, CA1 of hippocampus, frontal cortex and septum of Tph2−/− mice. (B) Binding density of 5-HT_1A receptors labeled by the radioligand [³H]WAY100635 was up-regulated in most of the brain regions of Tph2−/− mice. (C) 5-HT_1A receptor-mediated increase in [³⁵S]GTP-γ-S binding after stimulation revealed enhanced 5-HT_1A coupling in the frontal cortex and septum of Tph2−/− mice. (D) Binding density of 5-HT_1B receptors labeled by the radioligand [¹²⁵I]ICYP was also increased in some brain regions of Tph2−/− mice. For (B, C, D) results are expressed as optical density (OD = specific OD – nonspecific OD) and presented as means ± sem (n = 5). * indicates ANOVA significant output for genotype effect with *p<0.05, **p<0.01, ***p<0.001. For detailed statistical results see Table S1. c: cortex, Ss: somatosensory, Retrospl.: retrosplenial, CA1: cornu ammonis area 1 of hippocampus, g: gyrus, D: dorsal, Enth.: enthorinal, Caud. put.: caudate putamen, V pal.: ventral pallidus, Globus pal.: globus pallidus, Hypo. lat.: lateral hypothalamus, D subic.: dorsal subicullum, Subst. nigra: substantia nigra.

https://doi.org/10.1371/journal.pone.0043157.g004

Testing 5-HT Synthesis by a Functional Assay.

In slices, application of Trp increases de novo synthesis of 5-HT leading to increase in extracellular 5-HT which, in turn, activates somatodendritic 5-HT_1A receptors thereby inhibiting serotonergic neuron activity [16]–[18]. To functionally test whether in Tph2−/− mice serotonergic neurons are capable to synthesize 5-HT, we studied the effect of the application of 30 and 100 µM Trp on the firing rate of serotonergic neurons. As shown in Fig. 5D and E, the superfusion of Trp decreased firing rate of serotonergic neurons recorded in DR slices from wt and Tph2+/− mice, but not of those from Tph2−/− mice, confirming the absence of Tph activity and complete loss of 5-HT synthesis capacity from Trp in these neurons. In addition, in Tph2−/− mice, application of 5-HTP (the Tph2 product and 5-HT precursor) reversibly silenced serotonergic neurons (Fig. 5D), indicating that 5-HT metabolism downstream of Tph2 and functional response of serotonergic neurons to endogenous 5-HT, when present, are preserved in Tph2−/− mice.

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Figure 5. Electrophysiological characteristics of serotonergic raphe neurons.

Tph2−/− mice displayed electrophysiological characteristics similar to +/− and wt mice comprising (A) mean firing rate of the recorded neurons measured over a 3 min interval; (B) Up-to-Downstroke Interval (UDI) measured as shown in (C); wt: n = 19; Tph2+/−: n = 25; Tph2−/−: n = 21. (D) Representative time-course of the effect of tryptophan (Trp 30 and 100 µM) and R-8-OH-DPAT (DPAT; 30 nM) application on the firing rate of serotonergic neurons in slices taken from wt (upper panel), Tph2+/− (middle panel) and Tph2−/− (lower panel) mice. Lower panel also illustrates the response of Tph2−/− mice to the application of 30 µM L-5-hydroxytryptophan (5-HTP). 5-HTP stopped the firing of serotonergic neurons in all three genotypes. (E) Bar graph summarizes the responses to Trp application shown in (D). Both concentrations of Trp (30 and 100 µM) did not change firing of serotonergic neurons in Tph2−/−, but significantly inhibited serotonergic neuron firing in wt and Tph2+/− mice (p<0.05, Wilcoxon Signed Rank Test). When compared across genotypes, the responses of Tph2−/− serotonergic neurons were statistically different from those of +/− and wt mice both for Trp 30 µM (H₍₂₎ = 16.28, p<0.0003, wt n = 7; Tph2+/− n = 10; Tph2−/− n = 12) and 100 µM (H₍₂₎ = 10.43, p = 0.0054, wt n = 7; Tph2+/− n = 8; Tph2−/− n = 8; Kruskal-Wallis, followed by Dunn’s multiple comparison test). Diagram bars represent means ± SD.

https://doi.org/10.1371/journal.pone.0043157.g005

Growth Retardation and Dual Dose-dependent Body Weight Regulation

While a normal overall life expectancy was observed for adult Tph2-deficient mice, both male and female Tph2−/− mice consistently displayed leanness with lower body weights than their littermates. Hypomorphism was observed during the early developmental period and persisted across the entire lifespan reflecting reduced fat storage. This reduction of body weight may result from altered regulation at different levels, including reduced food intake, implicating impaired perception of energy needs and satiety; increased metabolic activity and energy expenditure or lower storage, implicating dysregulated glucose, lipid and protein metabolic cycles or thermoregulation. Growth retardation in another mouse line of Tph2−/− mice was previously observed but with a catch-up starting after weaning and normal weight reached at age 4 months [19]; however, these mice differ from ours by their genetic background and older mice were not studied. Consistent with our findings, other authors reported persistent low body weight in Tph2−/− mice at 24 and 48 weeks associated with a reduced fat pad and size and with both reduced food intake and increased metabolism linked to altered leptin regulation [20].

The leanness of 5-HT deficient mice was unexpected in the face of reports that 5-HT or drugs increasing its release are classically found anorexigenic via hypothalamic actions [21], reducing meal size and body weight [21]–[25] and increasing energy expenditure [26]. While the low body weight of Tph2−/− contrasts previous findings and conclusions, observation of obesity in Tph2+/− (exhibiting reduced brain 5-HT) as reflected by excess abdominal and pericardial fat storage, particularly in Tph2+/− females beyond 24 weeks, concurs. Similar complex dual roles of 5-HT was suggested by studies in the nematode C. elegans [27]. In mice, 5-HT transporter null mutants (5-Htt−/−), which display increased synaptic 5-HT but a reduced synthesis and total 5-HT brain concentrations [28] also develop obesity in adulthood [29], [30]. In summary, the sex- and gene dose-dependent divergent body weight and fat storage phenotypes in Tph2 mutant mice supports the notion of a nonlinear dual effect of central 5-HT on somatic development, long-term body weight regulation, and metabolic homeostasis via different pathways interacting with hormonal status.

5-HT Deficiency and Impact on other Neurotransmitter Systems

In Tph2−/− mutants 5-HT concentrations are dramatically reduced across brain regions and virtually absent from the serotonergic neuron-containing raphe region with only traces detectable by HPLC, demonstrating that 5-HT synthesis within neurons depends on the activity of the Tph2 isoform. While perfusion of brain with removal of most of the residual blood in capillaries resulted in minimal amounts of 5-HT in rostral raphe region at the lower detection limit (<1.2% in Tph2−/−), it is still likely that a few blood cells with high 5-HT content, such as platelets or mastocytes, remain trapped in capillaries or that blood diffused post-mortem in brain tissue. Very low brain 5-HT levels were also detected in other Tph2−/− mice [19] as well as in Tph1/Tph2−/− double-knockout mice [31]. In addition, we previously showed that Tph1 is not upregulated in Tph2−/− brain indicating that Tph1-driven synthesis can be ruled out in the brain [7]. However, there are several alternative explanations for the remaining traces: 1) HPLC does not detect 5-HT in Tph2-deficient mice but a closely related compound with the same retention time, a possibility which could be resolved by mass spectrometry, 2) minimal amount of the immediate 5-HT precursor, 5-HTP, produced by peripheral Tph1 crosses the blood brain barrier and can be transformed into 5-HT since AADC is ubiquitously expressed, 3) other enzymes, such as phenylalanine hydroxylase, or as yet unknown enzymes, use Trp as substrate and produce a small amount of 5-HT, 4) alternative metabolic pathways are able to produce 5-HT as end- or by-product. Of note, 5-HIAA is more reduced, or even undetectable, than 5-HT itself suggesting that either the metabolic pathway of 5-HT is inhibited, with MAOA activity specifically down-regulated in 5-HT neurons, or the 5-HT-like traces do not represent 5-HT but another compound degraded via another pathway. Taken together, the deficiency in 5-HT availability is so extreme that we assume that 5-HT neurotransmission is abolished in Tph2−/− brain despite the presence of neurons with serotonergic cell-like specification.

While DA concentrations are only reduced in Hip, 5-HT deficiency is accompanied by a consistent reduction of NE across brain regions. Tph2−/− mice exhibited a reduced number of TH expressing cells in some subparts of the LC which can partly explain the lower NE content in its projection areas. The LC is extensively innervated by Sert-positive fibers containing 5-HT in wt controls and devoid of 5-HT in Tph2−/− mice. We hypothesize that the absent trophic effect of 5-HT in Tph2−/− mice impacts development or survival of NE-specific neurons. Alternatively, absence of 5-HT release prevents the stimulation or inhibits, presumably by indirect input from inhibitory GABAergic or excitatory glutamatergic neurons, expression and activity of TH in NE neurons. Several studies reported that chronic treatment with the SSRI fluoxetine induces an increase of TH gene expression in the LC [32]. Conversely, 5-HT deficiency may thus down-regulate TH activity in the LC, eventually reducing NE biosynthesis. TH is also present along NE fibers projecting towards target areas and regulation at the level of terminals is likely since the DR does not seem to exert a direct inhibitory influence on the release of NE in the LC [33]. While 5-HT and NE fibers with synaptic varicosities colocalize in forebrain regions, a feedback loop involving alpha-2 adrenergic receptors on 5-HT fibers and 5-HT₃ receptors on NE fibers, allows a reciprocal regulation of release of both neurotransmitters by which 5-HT₃ receptors stimulate the synaptic release of NE [34]. The stimulation of the neurotransmitter release is accompanied by an activation of its synthesis, whereas the lack of stimulating effect by 5-HT on NE fibers dampens TH activity and thus NE synthesis. The interaction between serotonergic and noradrenergic systems have attracted attention as both systems are implicated in the control of a wide range of complex behaviors as well as the pathogenesis of affective disorders and their treatment by dual 5-HT/NE reuptake inhibitors (SNRI) [35]. Although moderate increase of brain NE content was observed in other Tph2−/− mice [20], the NE concentrations reported in [19] and [31] tend to be reduced but the difference did not reach significance. Overall, the findings confirm that serotonergic and noradrenergic systems are interdependent and subject to co-regulation involved in behavior and psychopathology.

Molecular Specification of Raphe Neurons Lacking 5-HT Synthesis

Although considerable evidence supports morphogenetic properties of 5-HT [3], [4] regulating proliferation, migration and differentiation of neural cells, we did not observe gross neuroanatomical alteration in the brain of Tph2−/− mice. One of the aims of the present study was to elucidate whether expression of genes specifying a serotonergic phenotype is conserved in raphe neurons lacking 5-HT synthesis. We demonstrated that Sert is present on the soma of raphe neurons as well as on their fibers and terminals in the various projection areas, although they had lost the capacity to synthesize and thus release 5-HT (Fig. 3 and [5]). In addition to Sert, the serotonergic cell-specific transcription factor Pet1, the monoaminergic-specific Vmat2 as well as 5-HT_1A functioning as somatodendritic autoreceptors, are expressed by neurons displaying a 5-HT neuron-like morphological phenotype in Tph2−/− mice. Finally, the 5-HT devoid neurons exhibit typical electrophysiological properties of pacemaker firing and are able to produce 5-HT via AADC if supplemented with 5-HTP, suggesting a functional 5-HT synthesis pathway downstream Tph2. While the genetic inactivation of the upstream transcription factors Lmx1b and Pet1 compromises the development of the majority of 5-HT neurons [11]–[13], we conclude that intrinsic 5-HT production is neither essential for the development, differenciation, maintenance and survival of serotonergic neurons, nor for the molecular specification of a serotonergic-like phenotype. It remains, however, to be elucidated in detail whether subtle alteration in dendritic arborization, neurite target finding, or brain structures innervation occurs and whether serotonergic neurons use neuropeptides and/or other monoamines with low affinity for the Sert as physiological or “borrowed” neuromodulator or transmitter in establishing function and connectivity.

Adaptive 5-HT_1A and 5-HT_1B Receptors Regulation

The density of 5-HT_1A and 5-HT_1B receptors and their G-protein coupling were significantly increased across many brain regions of 5-HT deficient Tph2−/− male mice, particularly in terminal fields of the FC and Sep. These findings are in accordance with an early study showing that complete abolition of 5-HT synthesis by p-chlorophenylalanine (PCPA) treatment led to significant up-regulation of 5-HT_1A and 5-HT_1B receptor binding sites evaluated in cerebral cortex areas [36]. The opposite phenomenon was observed in mouse models characterized by robust increases of extracellular 5-HT in the brain such as MAO-A null mutant mice where 5-HT_1A and 5-HT_1B receptors are desensitized and down-regulated [37], [38] and, to a lesser extent and in a brain region specific manner, in 5-Htt−/− mice [39], [40]. Interestingly, 5-HT_1A receptors are down-regulated in patients with depression and anxiety disorders as well as during SSRI treatment [41]–[43]. Sensitization and up-regulation of 5-HT_1A and 5-HT_1B receptors in 5-HT deficient mice may likely be due to direct cellular and molecular mechanisms compensating for reduced 5-HT ligand availability by an increase of Htr1a and Htr1b gene expression in target neurons, resulting in increased receptors production and thus increased binding site densities.

5-HT Deficient Neurons Retain their Electrophysiological Properties

In Tph2−/− mice, serotonergic raphe neurons appear morphologically conserved and express all known markers of serotonergic specification (except Tph2 and 5-HT). In brainstem slices obtained from Tph2−/− mice, serotonergic neurons also retained the typical slow (1–2 spikes/s) tonic firing pattern which, together with the preserved shape of the action current, indicates that this spontaneous firing is independent from endogenous 5-HT synthesis and moreover that the absence of 5-HT did not produce adaptive changes of voltage-sensitive membrane channels responsible for the pacemaker activity. Relevant to the functional effectiveness of Tph2 gene deletion, the fact that Trp did not inhibit the firing of serotonergic neurons clearly shows that synthesis of neuronal 5-HT is mediated exclusively by the Tph2 isoform and that it is abolished in the neurons of Tph2−/− mice. However, when in slices from Tph2−/− mice, the Tph2-dependent step of 5-HT synthesis is bypassed by supplementation with intermediary 5-HTP (that is converted into 5-HT by AADC) a robust 5-HT_1A autoreceptor-mediated inhibition of neuron firing is revealed, showing that responsiveness of serotonergic neurons to 5-HT persists in Tph2−/− neurons. This finding, together with the preserved response to the selective 5-HT_1A agonist DPAT, shows that 5-HT_1A receptors are functional and confirms that the lack of response of serotonergic neurons to Trp in Tph2−/− mice is indeed due to the lack of Tph2 and not to the absence of functional 5-HT_1A-mediated autoinhibition or their downstream effectors. In brainstem slices from Tph2+/− mice, serotonergic neurons responded to Trp application with a decrease in firing rate that was similar to that observed in wt mice, showing that gene dose dependent reduction of 5-HT synthesis does not result in functional changes in the 5-HT system at baseline. Overall, electrophysiological data provide evidence that endogenous 5-HT is not required for acquisition and preservation of the functional properties typical of serotonergic neurons.

Conclusion and Perspectives

This study examined the consequence of brain 5-HT deficiency from the earliest stage of ontogeny on somatic growth, brain development and the differentiation of the serotonergic system itself at various levels. First, we found that brain 5-HT has a dose-dependent dual nonlinear effect on the regulation of body weight probably involving distinct mechanisms interacting with hormonal system. Although, in vitro and in vivo previous studies, using neurotoxins, SSRIs or genetically driven 5-HT alterations [3], [4], [44], indicated a morphogenetic developmental role for 5-HT during brain development, Tph2−/− mice are viable and their brain appears structurally normal except a moderate reduction of NE neurons number. Since the mice investigated here were all born to Tph2+/− mothers with limited 5-HT loss in the brain (and normal level in the periphery), and the placenta is able to produce 5-HT (e.g. [45]), it is possible that Tph2−/− embryos’ brain received during early prenatal development and until their blood brain barrier (BBB) is closed, sufficient 5-HT of exogenous origin (peripheral, placental or maternal) to pursue virtually normal prenatal development, however, a complete compensation is still unlikely. The critical developmental window during which 5-HT signaling deficiency have deleterious effects on brain development is thus in the early phase of development and is independent of endogenous neuronal 5-HT synthesis. Given that Tph2, but nether Tph1, is expressed in the mouse brain from embryonic day (E) 10–10.5 [7], [46], the critical period might be likely before E10, however, a role for exogenous 5-HT is still possible until the BBB is closed. This underlines that, if 5-HT is necessary for normal brain development, exogenous 5-HT, most likely Tph1-derived and of maternal, placental and/or peripheral origin, is the critical source for early prenatal brain development [46]. Alternatively, it seems that, except for the use of neurotoxins which might have other 5-HT-independent toxic side-effects, an excess of 5-HT signaling (in 5-Htt−/− or SSRI treatments) has more deleterious effects on brain development than 5-HT deficiency. Nevertheless, in adult age, 5-HT synthesis deficiency results in behavioral alterations including in response to environmental stressors (Gutknecht et al., unpublished data), reduces the function of other monoaminergic systems and may also have an impact on brain repair in case of injury. Our results also demonstrate that the proper cellular and molecular differentiation and the maintenance of the serotonergic neuron phenotype do not require endogenous 5-HT synthesis. Moreover, serotonergic neurons continue to display characteristic spontaneous firing although they are devoid of 5-HT showing that this electrophysiological mechanism depends on programming independent from 5-HT synthesis and release. Finally, it remains to be determined whether serotonergic neurons play a physiologically relevant role independent from 5-HT neurotransmission and via which pathway(s). Such supplementary signaling would equip serotonergic neurons with an as yet unknown parallel function.

Animals and Ethics Statement

All animal manipulations were approved by the review board of the government of lower Franconia and the University of Wuerzburg, and performed according to the European Community guidelines for animal care (Permit number: DL 116/92, application of the European Communities Council Directive 86/609/EEC). A maximum effort was made to minimize the number of animals used and their suffering - see also Supporting Information (SI) (Text S1, Supplemental Materials and Methods). The generation and genotyping procedure of Tph2−/− animals have been described in [5]. Their genetic background is composed theoretically of 97% C57BL/6N and 3% Sv129/Ola.

Body Weight Across the Lifespan

Body weight was determined in different animals at different ages from 3 weeks up to 2.2 years. The weighted cohort included 421 males: 155 wt, 173+/− and 93−/− and 374 females: 147 wt, 146+/− and 81−/−. Age was used as covariable in the ANOVA comparison between genotypes within each sex.

Brain Neurotransmitters Concentrations

Two independent cohorts of 4 months old mice were used. The first one was composed of 8 Tph2−/− (mixed 4 males and 4 females because of the low number of −/− animals at that time), 8+/− males and 8 wt males which were perfused for 10 min with PBS. Since no sex effect could be observed, Tph2−/− mice were subsequently pooled in the graphs and analyses. ANOVA requirements failed in a majority of cases, therefore, we applied to all non-parametric analysis of variance. Because the efficiency of the brain perfusion was not ideal and blood traces were still visible in the brain of the first animals, a second cohort composed exclusively of males with 4 Tph2−/−, 4+/− and 4 wt, was used to reanalyze 5-HT concentrations in brain regions. These animals were this time perfused for 10 min at higher pump debit with PBS containing 20 U/ml Heparin. Perfusion was obviously better and brains were visibly whiter. For both cohorts, brains were immediately frozen until brain regions were dissected and neurotransmitters concentrations analysed using HPLC as described in SI (Text S1).

Histological Staining

Immunohistochemical stainings were performed on brains fixed by perfusion with 4% paraformaldehyde, cryoprotected, frozen and sliced into 14 µm sections. After epitope retrieval and blocking, the primary antibodies against Tph2, Sert and TH were applied, followed by incubation with biotinylated secondary antibodies. Staining was revealed using the Avidin-Biotin Complex method with diaminobenzidine as chromogene. Double-fluorescent 5-HT and Vmat2 primary antibodies immunostaining were realized by applying fluorescent Alexa fluor 488- and Dylight-conjugated secondary antibodies respectively. For Pet-1 in situ hybridization, 16 µm sections from native frozen brains were used. Digoxigenin (DIG) labeled Pet-1 cRNA probes were applied to brain sections and visualized by alkaline phosphatase conjugated anti-DIG antibody. The number of TH-immunoreactive cells was quantified from 8 mice of each genotype. Detailed protocols are described in SI (Text S1).

Receptors Binding and Stimulation

A cohort of n = 5 males per genotype, 5 months old, were used. Frozen brains were entirely sectioned at 16 µm and spread in 8 adjacent sections series for 5-HT_1A and 5-HT_1B receptors specific binding and stimulation experiments, as well as the respective non-specific controls.

Specific binding to 5-HT_1A was performed with 2 nM [³H]-WAY100635 and with 12 pM [¹²⁵I]-Cyanopindolol for 5-HT_1B. For both receptors, nonspecific binding was estimated from adjacent sections incubated in the same medium supplemented with 10 µM 5-HT. Results are expressed as specific binding OD - nonspecific OD. Agonist-stimulated binding of 0.05 nM [³⁵S]-GTP-γ-S was performed with 10⁻⁷ M 5-CT. Nonspecific binding was determined from adjacent sections in presence of 1 nM of antagonist WAY100635 for 5-HT_1A or GR127935 for 5-HT_1B.

Electrophysiological Recording of Raphe Neurons

Mice (28 to 80 days old) were anaesthetized with isofluorane and decapitated. The brain was rapidly removed, dissected in ice-cold gassed (95% O₂ and 5% CO₂) artificial cerebrospinal fluid (ACSF) containing (in mM): 124 NaCl, 2.75 KCl, 1.25 NaH₂PO₄, 1.3 MgCl₂, 2 CaCl₂, 26 NaHCO₃, 11 D-glucose (pH 7.4), and the brainstem was sliced coronally into 200 µm thick slices with a vibratome. After recovery, the slices were individually transferred into the recording chamber and superfused continuously with warmed ACSF (34–35°C) at a rate of 2 ml min⁻¹. Neurons were visualized by infrared differential interference contrast video microscopy with a Newicon C2400-07 camera (Hamamatsu, Hamamatsu City, Japan) mounted to an Axioskop microscope (Zeiss, Göttingen, Germany). Recordings were made using an EPC-10 amplifier (HEKA Elektronic, Lamberecht, Germany). Patch pipettes were prepared from thick-walled borosilicate glass on a P-97 Brown-Flaming electrode puller (Sutter Instruments, Novato, CA) and had resistance of 3–6 MΩ when filled with solution containing (in mM): 125 NaCl, 10 HEPES, 2.75 KCl, 2 CaCl₂, 1.3 MgCl₂ (pH 7.4 with NaOH). Loose-seal cell-attached recordings (5–20 MΩ seal resistance) were acquired continuously in voltage-clamp mode. Signals were filtered at 3 kHz and digitized at 10 kHz. Pipette potential was maintained at 0 mV. Recordings were aborted if firing rate was sensitive to changes in pipette holding potential or if shape of action current changed. Data were analyzed using Clampfit 9.2 (Molecular Devices) and Prism 5 (GraphPad Software, San Diego, CA). Extracellular saline was supplemented with 10 µM phenylephrine to facilitate firing. Neurons were presumed serotonergic when 1) displayed firing rate of less than 3.5 Hz, 2) had asymmetric action current with peak-to-peak interval greater than 1 ms, and 3) their firing stopped in response to application of the 5-HT_1A receptor agonist DPAT (30 nM) at the end of experiment. Since experiments depended on endogenous 5-HT, recordings were done from neurons located at least 50 µm below the slice surface [18]. One experiment was done in each slice. The number of used mice and recorded cells for each particular design is indicated in results section and Fig. 5 legend.

Statistical Analysis

Unless otherwise specified, such as for electrophysiological data analysis (see Figure legend), the effects of genotype and sex were analyzed using ANOVA (indicated by F_(df1,df2) values) followed up with Tuckey-HSD post hoc tests for multiple group comparison. When requirements for one-way ANOVA (normal distribution, equality of variances) were not fulfilled, non-parametric Kruskall-Wallis analysis of variance was applied (indicated by H_(df) values), followed by Mann-Whitney-U-Test. P<0.05 was considered statistically significant and 0.05<p≤0.10 was considered as a trend of significance.