Topic Introduction

Preparation and Use of Retroviral Vectors for Labeling, Imaging, and Genetically Manipulating Cells

  1. Fred H. Gage
Adapted from Imaging: A Laboratory Manual (ed. Yuste). CSHL Press, Cold Spring Harbor, NY, USA, 2011.

Abstract

Retroviral vectors are a powerful technology for achieving long-term genetic manipulation. This introduction provides some background on replication-deficient retroviral vectors based on Moloney murine leukemia virus and lentivirus. Details, examples, and associated protocols are provided for using these vectors to fluorescently label, genetically alter, and image both live and fixed murine brain tissue.

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  1. doi:10.1101/pdb.top086363 Cold Spring Harb Protoc 2015: pdb.top086363- © 2015 Cold Spring Harbor Laboratory Press
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