Protocol

Purification and Injection of Retroviral Vectors

  1. Fred H. Gage
Adapted from Imaging: A Laboratory Manual (ed. Yuste). CSHL Press, Cold Spring Harbor, NY, USA, 2011.

Abstract

Retroviral vectors are powerful tools for genetic manipulation. This protocol discusses the production, purification, and use of replication-deficient retroviral vectors based on Moloney murine leukemia virus and lentivirus. It also describes the injection of a retroviral vector into the dentate gyrus of young adult mice to fluorescently label live murine brain tissue.

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  1. doi:10.1101/pdb.prot086371 Cold Spring Harb Protoc 2015: pdb.prot086371- © 2015 Cold Spring Harbor Laboratory Press
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