Basic–alimentary tractSex Steroid Regulation of Macrophage Migration Inhibitory Factor in Normal and Inflamed Colon in the Female Rat
Section snippets
Animals
Adult female Wistar rats (Charles River, Saint-Aubin-Lès-Elbeuf, France) were housed in groups of 4 with free access to food and water, under a 12 hour:12 hour light:dark cycle. All protocols were approved by the local institutional Animal Care and Use Committee, in compliance with the European laws on the protection of animals (86/609/EEC).
Determination of Estrous Cycle Stages and Ovariectomy
In a first experiment, estrous cycle stages were assessed through daily vaginal smears, as previously described.30 Only rats displaying regular cycles were
MIF, IL-1β, and TNF-α Colonic Contents During the Estrous Cycle, and Effect of Ovariectomy
MIF concentrations increased significantly from the follicular to the luteal phase of the cycle (Figure 1A) (301 ± 39 and 781 ± 135 ng/mg of protein in estrus and metestrus, respectively; P < .05). In the same rats, no significant variation was observed throughout the cycle for IL-1β (range, 28 ± 3 to 38 ± 5 pg/mg of protein) and TNF-α (range, 3.6 ± 0.1 to 4.1 ± 0.3 pg/mg of protein) (Figure 1B and C).
In OVX rats, MIF concentrations were decreased (117 ± 28 vs. 628 ± 85 ng/mg of protein in the
Discussion
The current study shows that female sex hormones are involved in the control of MIF expression in the normal and inflamed colon. In IBD and animal models of colitis, MIF is a proinflammatory cytokine that stimulates the production of TNF-α and IL-1β,20, 21 both playing a central role in the initiation and perpetuation of mucosal inflammation.37, 38, 39 Our study in female rats provides evidence of a relationship between basal MIF levels, estrous cycle-dependent hormonal status, and the capacity
References (61)
- et al.
Gynecologic history of women with inflammatory bowel disease
Obstet Gynecol
(1995) - et al.
The menstrual cycle and its effect on inflammatory bowel disease and irritable bowel syndrome: a prevalence study
Am J Gastroenterol
(1998) - et al.
Progesterone, but not 17β-estradiol, increases TNF-α secretion in U937 monocytes
Cytokine
(2004) - et al.
Ubiquitous distribution of macrophage migration inhibitory factor by human gastric and intestinal epithelium
Gastroenterology
(2002) - et al.
Amelioration of dextran sulfate sodium-induced colitis by anti-macrophage migration inhibitory factor antibody in mice
Gastroenterology
(2002) - et al.
The immune response during the luteal phase of the ovarian cycle: increasing sensitivity of human monocytes to endotoxin
Fertil Steril
(2001) - et al.
Progesterone and allopregnanolone reduce inflammatory cytokines after traumatic brain injury
Exp Neurol
(2004) - et al.
Macrophage migration inhibitory factor: a central regulator of wound healing
Am J Pathol
(2005) - et al.
Quantitative assay for acute intestinal inflammation based on myeloperoxidase activityAssessment of inflammation in rat and hamster models
Gastroenterology
(1984) - et al.
Progesterone in conjunction with estradiol has neuroprotective effects in an animal model of neurodegeneration
Pharmacol Biochem Behav
(1999)
Vagally dependent protective action of calcitonin gene-related peptide on colitis
Peptides
Central effects of selective NK1 and NK3 tachykinin receptor agonists on two models of experimentally-induced colitis in rats
Peptides
Role of tumor necrosis factor receptors in an animal model of acute colitis
Cytokine
Gender-related effects of chronic non-malignant pain and opioid therapy on plasma levels of macrophage migration inhibitory factor (MIF)
Pain
Macrophage migration inhibitory factor induces cell death and decreases neuronal nitric oxide expression in spinal cord neurons
Neuroscience
Oestrogen receptor beta (ERbeta) is abundantly expressed in normal colonic mucosa, but declines in colon adenocarcinoma paralleling the tumour’s dedifferentiation
Eur J Cancer
Macrophage migration inhibitory factor promotes intestinal tumorigenesis
Gastroenterology
Hormone replacement therapy and the risk of colorectal cancer: a meta-analysis
Obstet Gynecol
Involvement of interleukin-1 in the development of ulcerative colitis induced by dextran sulfate sodium in mice
Cytokine
Tumor necrosis factor α and interleukin 1β in relapse of Crohn’s disease
Lancet
Mechanisms in failure of infliximab for Crohn’s disease
Lancet
Efficacy and safety of retreatment with anti-tumor necrosis factor antibody (infliximab) to maintain remission in Crohn’s disease
Gastroenterology
Macrophage migration inhibitory factor is an important mediator in the pathogenesis of gastric inflammation in rats
Gastroenterology
Influence of sex and disease on illness-related concerns in inflammatory bowel disease
Can J Gastroenterol
Health-related quality of life in patients with inflammatory bowel disease five years after the initial diagnosis
Scand J Gastroenterol
The epidemiology of inflammatory bowel disease in Canada: a population-based study
Am J Gastroenterol
Premarin-induced ischemic colitis
J Clin Gastroenterol
Risk factors for inflammatory bowel disease in the general population
Aliment Pharmacol Ther
Estradiol treatment ameliorates acetic acid-induced gastric and colonic injuries in rats
Inflammation
Beneficial effects of estrogen treatment in the HLA-B27 transgenic rat model of inflammatory bowel disease
Am J Physiol Gastrointest Liver Physiol
Cited by (73)
T helper cell immunity in pregnancy and influence on autoimmune disease progression
2021, Journal of AutoimmunityDysregulation of cytokine coding genes in peripheral blood of bipolar patients
2019, Journal of Affective DisordersCitation Excerpt :Benedek et al. have shown higher levels of this cytokine in males with progressive MS compared with female MS patients and suggested MIF as a sex-specific disease modifier (Benedek et al., 2017). This cytokine has both pro- and anti-depressant functions (Bloom and Al-Abed, 2014) and is recognized to have sex-specific functions in inflammation, wound healing and response to ischemic stroke (Houdeau et al., 2007; Turtzo et al., 2013). Notably, we demonstrated higher expression of CXCL8 in total patients, male patients and female patients compared with the corresponding control groups.
Do Sex Hormones Cause, or Are They Only Associated With, Microscopic Colitis?
2018, GastroenterologyBisphenol A—Induced changes in the enteric nervous system of the porcine duodenum
2018, NeuroToxicologyCitation Excerpt :It is known that this substance may alter the intestinal barrier function and gut nociception. Moreover, acting on the estrogen receptors, which are widely expressed in epithelial and immune cells of the digestive tract (Campbell-Thompson et al., 2001; Konstantinopoulos et al., 2003), BPA may be involved in processes associated with gastrointestinal inflammation (Harnish et al., 2004; Houdeau et al., 2007; Verdú et al., 2002). Some studies also suggest that perinatal or juvenile period exposure to xenoestrogens (including BPA) is a factor predisposing to inflammatory bowel disease, Crohn’s disease or ulcerative colitis in later life (for review, see Braniste et al., 2011).
Role of sex and aging in mucosal health
2018, Conn's Handbook of Models for Human AgingEx Vivo Colonic Tissue Susceptibility to HIV-1 in Cisgender Men and Women
2024, AIDS Research and Human Retroviruses
Supported by the Institut National de la Recherche Agronomique (INRA).