REVIEW ARTICLETargeted electroporation in Xenopus tadpoles in vivo – from single cells to the entire brain
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Quantifying neuronal structural changes over time using dynamic morphometrics
2022, Trends in NeurosciencesEarly Developmental Exposure to Fluoxetine and Citalopram Results in Different Neurodevelopmental Outcomes
2021, NeuroscienceCitation Excerpt :Inter-tadpole angles were measured by fitting a line through the tapdole’s body and calculating the angle relative to its neighbors (Lopez III et al., 2021). To label single tectal cells, stage 45–47 tadpoles were anesthetized and whole-brain electroporation was used to transfect tectal neurons with a fluorescent protein (Haas et al., 2002). Tadpoles were anesthetized with 0.02% MS-222 and, plasmid DNA was pressure injected into the brain’s middle ventricle using a glass micropipette.
Lhx2/9 and Etv1 Transcription Factors have Complementary roles in Regulating the Expression of Guidance Genes slit1 and sema3a
2020, NeuroscienceCitation Excerpt :The firefly luminescence was normalized against the Renilla luminescence and expressed as relative light units. Stage 27/28 embryos were anaesthetized in 0.4 mg/mL tricaine methanesulfonate (Sigma-Aldrich; Oakville, Canada) in 1 × modified Barth’s saline (0.7 mM CaCl2, 5 mM HEPES, 1 mM KCl, 1 mM MgSO4, 88 mM NaCl, 2.5 mM NaHCO3, pH 7.8) and the brains electroporated as described by Haas et al. (2002) (schematized in Fig. 2A). A Picospritzer II platform (General Valve) equipped with a borosilicate needle was used to inject 1 μg/μL DNA solution into the central brain ventricle.
In vivo imaging of synaptogenesis
2020, Synapse Development and Maturation: Comprehensive Developmental Neuroscience