Issue 15, 2013
From the journal:

Lab on a Chip

C.L.I.P.–continuous live imaging platform for direct observation of C. elegans physiological processes†

Jan Krajniak,a   Yan Hao,b   Ho Yi Makb  and  Hang Lu*acd  

* Corresponding authors

a School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, USA

b Stowers Institute for Medical Research, Kansas City, MO, USA

c Interdisciplinary Program in Bioengineering, Georgia Institute of Technology, Atlanta, GA, USA

d The Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, 311 Ferst Drive, N.W., Atlanta, GA 30332-0100, USA
E-mail: hang.lu@chbe.gatech.edu
Fax: +1 404 894 4200
Tel: +1 404 894 8473

Abstract

Direct observation of developmental and physiological changes in certain model organisms over time has been technically challenging. In the model organism Caenorhabditis elegans, these studies require frequent or continuous imaging at physiologically benign conditions. However, standard methods use anaesthetics, glue, or microbeads, which prevent animals from feeding during the experiment. Thus, the animals' normal physiological function may be affected over time. Here we present a platform designed for dynamic studies of C. elegans. The system is capable of immobilizing only the animals' bodies under benign conditions and without physical deformation. Simultaneously, the animals' heads remain free to move and feed for the duration of the experiment. This allows for high-resolution and high-magnification fluorescent imaging of immobilized and feeding animals. The system is very easy to fabricate, set up, and operate, and should be widely applicable to many problems in developmental and physiological studies.

Graphical abstract: C.L.I.P.–continuous live imaging platform for direct observation of C. elegans physiological processes

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Article information

DOI
https://doi.org/10.1039/C3LC50300C
Article type
Paper
Submitted
05 Mar 2013
Accepted
16 Apr 2013
First published
16 Apr 2013

Lab Chip, 2013,13, 2963-2971

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C.L.I.P.–continuous live imaging platform for direct observation of C. elegans physiological processes

J. Krajniak, Y. Hao, H. Y. Mak and H. Lu, Lab Chip, 2013, 13, 2963 DOI: 10.1039/C3LC50300C

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