Issue 5, 2010

Lifespan-on-a-chip: microfluidic chambers for performing lifelong observation of C. elegans

Abstract

This article describes the fabrication of a microfluidic device for the liquid culture of many individual nematode worms (Caenorhabditis elegans) in separate chambers. Each chamber houses a single worm from the fourth larval stage until death, and enables examination of a population of individual worms for their entire adult lifespans. Adjacent to the chambers, the device includes microfluidic worm clamps, which enable periodic, temporary immobilization of each worm. The device made it possible to track changes in body size and locomotion in individual worms throughout their lifespans. This ability to perform longitudinal measurements within the device enabled the identification of age-related phenotypic changes that correlate with lifespan in C. elegans.

Graphical abstract: Lifespan-on-a-chip: microfluidic chambers for performing lifelong observation of C. elegans

Supplementary files

Article information

Article type
Paper
Submitted
16 Sep 2009
Accepted
24 Nov 2009
First published
18 Dec 2009

Lab Chip, 2010,10, 589-597

Lifespan-on-a-chip: microfluidic chambers for performing lifelong observation of C. elegans

S. E. Hulme, S. S. Shevkoplyas, A. P. McGuigan, J. Apfeld, W. Fontana and G. M. Whitesides, Lab Chip, 2010, 10, 589 DOI: 10.1039/B919265D

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