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  • Original Paper
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Developmental expression of Pim kinases suggests functions also outside of the hematopoietic system

Abstract

We have cloned a novel quail cDNA with strong homology to the pim family of proto-oncogenes. The deduced amino acid (aa) sequence of the cDNA, named qpim, is more closely related to Xenopus Pim and to the recently identified rat Pim-3 than to human or rodent Pim-1 or Pim-2. The protein encoded by the qpim cDNA can autophosphorylate itself and share substrates with murine Pim-1, suggesting functional redundancy to other Pim family serine/threonine kinases. We have compared the expression of qpim in avian embryos to mouse pim-1, -2 and -3 by in situ hybridization. qpim shows a highly dynamic expression pattern, particularly at early developmental stages. Surprisingly, its expression pattern is not identical to any of the murine pim genes, which show complementary and/or partially overlapping expression sites both in- and outside of the hematopoietic system. Altogether, our results suggest novel functions for Pim family kinases during embryonic development, in particular in epithelia and in the central nervous system.

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Acknowledgements

We thank Nicole Le Douarin and Luc Pardanaud for critical reading of the manuscript, Anton Berns, Harald Mikkers and Steffan Ho for reagents, Tapio Tainola and Hannakaisa Laakkonen for expert technical assistance, Gerard Couly and other members of the Institut d'Embryologie for helpful discussions and Francoise Viala and Francis Beaujean for the illustrations. This study was supported by grants from Association pour la Recherche contre le Cancer and Ligue Nationale contre le Cancer to the Institut d'Embryologie (A Eichmann) and the Academy of Finland (PJ Koskinen).

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Eichmann, A., Yuan, L., Bréant, C. et al. Developmental expression of Pim kinases suggests functions also outside of the hematopoietic system. Oncogene 19, 1215–1224 (2000). https://doi.org/10.1038/sj.onc.1203355

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