Abstract
Cyclic AMP (cAMP) promotes neurite outgrowth in a variety of neuronal cell lines through the activation of protein kinase A (PKA). We show here, using both Xenopus laevis embryonic neuronal culture and intact X. laevis embryos, that the nerve growth–promoting action of cAMP/PKA is mediated in part by the phosphorylation of synapsins at a single amino acid residue. Expression of a mutated form of synapsin that prevents phosphorylation at this site, or introduction of phospho-specific antibodies directed against this site, decreased basal and dibutyryl cAMP–stimulated neurite outgrowth. Expression of a mutation mimicking constitutive phosphorylation at this site increased neurite outgrowth, both under basal conditions and in the presence of a PKA inhibitor. These results provide a potential molecular approach for stimulating neuron regeneration, after injury and in neurodegenerative diseases.
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Acknowledgements
This work was supported by U.S. Public Health Service Grants MH39327 (P.G.), AG15072 (P.G.), R29 NS35941 (V.P.) and NS37831 (M-m.P.). B.P. is a 2000 Katowitz-Raden Investigator of the National Alliance for Research in Schizophrenia and Affective Disorders. The catalytic subunit of PKA was a gift from A. Nairn and A. Horiuchi. We thank P. Allen for reviewing the manuscript, and G. Yiu and G. Chaiken for technical assistance.
*This work was also supported by U.S. Public Health Service Grants HG00008 (to J. Ott) and K25-HG00060-01A1 (J.H.)
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Kao, HT., Song, Hj., Porton, B. et al. A protein kinase A–dependent molecular switch in synapsins regulates neurite outgrowth. Nat Neurosci 5, 431–437 (2002). https://doi.org/10.1038/nn840
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DOI: https://doi.org/10.1038/nn840
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