Abstract
In mammals, odorant molecules are thought to activate only a few glomeruli, leading to the hypothesis that odor representation in the olfactory bulb is sparse. However, the studies supporting this model used anesthetized animals or monomolecular odorants at limited concentration ranges. Using optical imaging and two-photon microscopy, we found that natural odorants at their native concentrations could elicit dense representations in the olfactory bulb. Both anesthesia and odorant concentration were found to modulate the representation density of natural odorants.
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Acknowledgements
We thank I. Rodriguez for kindly providing the Omp-GFP mice. We thank I. Rodriguez, A. Holtmaat, S. Simon, M. Patterson and members of the Carleton laboratory for helpful discussions and comments on the manuscript. This work was supported by the Swiss National Science Foundation (SNF professor grant number PP0033_119169 and National Competence Center in Research “SYNAPSY”), the University of Geneva, the European Research Council (contract number ERC-2009-StG-243344-NEUROCHEMS), the Novartis foundation for medical research, the Leenaards foundation and the European Molecular Biology Organization (young investigator program).
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A.C., R.V., K.B., O.G. and J.B. carried out the study conceptualization and performed experiments or analysis. A.C., R.V. and O.G. wrote and edited the manuscript with comments from J.B. and K.B.
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Vincis, R., Gschwend, O., Bhaukaurally, K. et al. Dense representation of natural odorants in the mouse olfactory bulb. Nat Neurosci 15, 537–539 (2012). https://doi.org/10.1038/nn.3057
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DOI: https://doi.org/10.1038/nn.3057
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