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Laminar and compartmental regulation of dendritic growth in mature cortex

Nature Neuroscience volume 12, pages 116–118 (2009)Cite this article

Abstract

Can dendrites grow in mature cortex? We used chronic in vivo imaging to follow pyramidal neurons before and after cortical deletion of the Pten tumor suppressor gene in mature mice. We found that Pten/mTOR signaling uniquely regulates the growth of layer 2/3 apical dendrites; no effects of gene deletion were observed on basal dendrites of these pyramidal neurons or along layer 5 apical dendrites.

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Figure 1: Compartment-specific dendritic growth.
Figure 2: Dynamics of apical dendritic growth in vivo.
Figure 3: Effect of rapamycin.

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Acknowledgements

We thank E.M. Callaway and K. Bochmann for providing the ZnG reporter mice. This work was supported by the US National Eye Institute (EY016052), the Esther and Joseph Klingenstein Foundation, the University of California Los Angeles Center for Autism Research and Treatment, the US National Institute for Mental Health, the National Institute of Child Health and Human Development, the National Institute on Deafness and Other Communication Disorders, the National Institute of Neurological Disorders and Stroke (MH68172, University of California Los Angeles Studies to Advance Autism Research and Treatment Center) and a Ruth L. Kirchstein National Research Service Award (GM07185).

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Authors and Affiliations

  1. Department of Neurobiology, David Geffen School of Medicine, University of California Los Angeles, 635 Charles Young Drive, South, Los Angeles, 90095-1761, California, USA

    David K Chow & Joshua T Trachtenberg

  2. Institut du Fer a Moulin, INSERM U839, University Pierre & Marie Curie, 17 rue du Fer a Moulin, Paris, 75005, France

    Matthias Groszer

  3. Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, 650 Charles Young Drive, South, Los Angeles, 90095, California, USA

    Mochtar Pribadi & Xin Liu

  4. Department of Neurology, David Geffen School of Medicine, University of California Los Angeles, RNRC, Los Angeles, 90095-1769, California, USA

    Michal Machniki & S Thomas Carmichael

Authors
  1. David K Chow
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  2. Matthias Groszer
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  3. Mochtar Pribadi
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  4. Michal Machniki
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  5. S Thomas Carmichael
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  6. Xin Liu
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  7. Joshua T Trachtenberg
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Contributions

D.K.C., M.G. and J.T.T. designed the experiments. D.K.C. and J.T.T. carried out all of the imaging experiments, analyzed the data and wrote the manuscript. M.P. performed the pS6 immunostaining. M.M. and S.T.C. carried out the laser-capture quantitative real-time PCR. M.P. and X.L. provided the mice.

Corresponding author

Correspondence to Joshua T Trachtenberg.

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Supplementary Figures 1–3 and Supplementary Methods (PDF 11890 kb)

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Chow, D., Groszer, M., Pribadi, M. et al. Laminar and compartmental regulation of dendritic growth in mature cortex. Nat Neurosci 12, 116–118 (2009). https://doi.org/10.1038/nn.2255

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