Abstract
Neurotransmission operates on a millisecond timescale but is changed by normal experience or neuropathology over days to months. Despite the importance of long-term neurotransmitter dynamics, no technique exists to track these changes in a subject from day to day over extended periods of time. Here we describe and characterize a microsensor that can detect the neurotransmitter dopamine with subsecond temporal resolution over months in vivo in rats and mice.
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Acknowledgements
We thank S. Barnes for technical assistance and M. Walton and W. Shain for useful discussions. This work was supported by the University of Washington Royalties Research Fund and the US National Institutes of Health (R01-MH079292 to P.E.M.P.; R21-DA024140 to P.E.M.P.; and R01-DA014486 to N.S.). J.J.C. was supported by F32-DA024540, M.J.W. was supported by T32-AA007455 (to M. Larimer), J.O.G. was supported by T32-GM007270 (to D. Kimelman), and A.S.H., J.G.P. and E.A.H. were supported by T32-DA007278 (to C. Chavkin).
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P.E.M.P. conceived the work; S.B.E. optimized the microsensor design; J.J.C., S.G.S., N.S. and P.E.M.P. designed experiments and prepared the manuscript; J.J.C., S.G.S., M.J.W., J.O.G., E.A.H., A.S.H., J.G.P., C.A.A., I.W. and V.M. collected and analyzed data.
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Clark, J., Sandberg, S., Wanat, M. et al. Chronic microsensors for longitudinal, subsecond dopamine detection in behaving animals. Nat Methods 7, 126–129 (2010). https://doi.org/10.1038/nmeth.1412
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DOI: https://doi.org/10.1038/nmeth.1412
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