Characterization of the bombesin-like peptide receptor family in primates
Section snippets
Search for mammalian BB4R
RT-PCR and genomic PCR were conducted on rhesus, Xenopus, and human brain cDNA or genomic DNA using primers for the reported rhesus BB4R DNA fragment [10]. The primers were almost perfectly conserved with the Bombina BB4R DNA sequence. Unexpectedly, an amplified fragment from Xenopus cDNA and genomic DNA that perfectly matched the partial rhesus BB4R sequence [10] was found. Attempts to detect the partial rhesus BB4R by RT-PCR using human and rhesus mRNA samples were not successful. These
Poly(A)+ mRNA and genomic DNA isolation
Poly(A)+ mRNAs were isolated from rhesus hypothalamus, cerebellum, pituitary gland, pons, medulla oblongata, liver, brain (except for hypothalamus, cerebellum, pituitary gland, pons, medulla oblongata), liver, and testis using FastTrack 2.0 (Invitrogen, Carlsbad, CA, USA). Genomic DNA was purified from rhesus liver and Xenopus laevis brain. Human poly(A)+ RNAs and genomic DNA were purchased from Clontech (Palo Alto, CA, USA).
Genomic and RT-PCR for BB4R
Primers were based on a rhesus BB4 partial sequence [10] and had high
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Discovery of oridonin as a novel agonist for BRS-3
2022, PhytomedicineBombesin-like receptor 3 (Brs3) expression in glutamatergic, but not GABAergic, neurons is required for regulation of energy metabolism
2017, Molecular MetabolismCitation Excerpt :Mammalian BRS-3 may be conserved during evolution because it can modify the response pattern of other GPCRs or contribute to basal signaling [11]. Brs3 mRNA and BRS-3 binding activity are located in restricted regions of the brain, including portions of the hypothalamus, amygdala, and thalamus [12–19]. BRS-3 is also reported to be present at low levels in peripheral sites including pancreatic islets, developing testis, female reproductive tract, lung, and muscle, and in certain cancers [5,20–22] (https://gtexportal.org/home/gene/BRS3).
Gastrin-releasing peptide facilitates glutamatergic transmission in the hippocampus and effectively prevents vascular dementia induced cognitive and synaptic plasticity deficits
2017, Experimental NeurologyCitation Excerpt :There is a possible reason that NMB and GRP are differentially distributed in the rat nervous system which suggests that these 2 structurally related peptides may have very distinct functions as neuropeptides in the rat nervous system (Moody et al., 1986). In addition, it has been recognized that NMBR has greater than a 100-food higher affinity for NMB than GRP, whereas the GRPR has a greater than 50-fold higher affinity for GRP than NMB (Jensen et al., 2008; Sano et al., 2004) which might be an explanation of more effective in improving learning and memory of NMB than GRP. And other studies also found that GRP and NMB could exert not exactly the same physiological action in anxiety and fear-related behavior (Bedard et al., 2007).