Cognitive, Behavioral, and Systems NeuroscienceResearch PaperDifferential involvement of medial prefrontal cortex and basolateral amygdala extracellular signal-regulated kinase in extinction of conditioned taste aversion is dependent on different intervals of extinction following conditioning
Section snippets
Animals
Male Long-Evans rats (8 weeks old) were purchased from the Animal Center of the National Science Council, Taipei, Taiwan, and weighed 350–450 g. The animals were housed individually in metal cages (30×30×20 cm3) maintained in a temperature (22±1°C)- and humidity (55±1%)-controlled room under a 12-h/12-h light/dark cycle (lights on at 5:00 h). Rodent chow was available ad libitum. All rats were subjected to 23 h water deprivation prior to adaptation training. All experimental procedures were
Adaptation training and extinction processes
Before performing the reinstatement experiment, the average fluid consumption of the five water trials in the 5H (n=11) and 24H (n=10) groups during the adaptation training was 7.9±0.4 ml and 7.7±0.3 ml, respectively (Fig. 1B). The average sucrose solution consumption during the first extinction trial was 3.8±0.6 ml in the 5H group and 3.3±0.7 ml in the 24H group. In our previous experiments, animals subjected to non-reinforced sucrose pre-exposure (n=11) drank approximately 14–16 ml of the
Discussion
The reinstatement experiment in the present study demonstrated that extinction training beginning 24 h after the conditioning trial did not affect the expression of extinguished conditioned responding in the 24H group, whereas no reappearance of conditioned responding was observed in the 5H group when the extinction procedure was initiated 5 h after acquisition. Our behavioral results with CTA are consistent with Myers et al. (2006) and Kimura et al. (2008), demonstrating that the time interval
Conclusion
In summary, our results indicate that the original conditioning can be retained or inhibited by CTA extinction depending on the time interval between acquisition and extinction. The ERK transduction pathway in the mPFC and BLA is differentially involved in these processes.
Acknowledgments
We are grateful to Dr. Linda Chia-Hui Yu for valuable discussion. This study was supported by a grant from the National Science Council (NSC 97-2410-H002-214), ROC.
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