NeuroanatomyResearch PaperDivergent impact of the polysialyltransferases ST8SiaII and ST8SiaIV on polysialic acid expression in immature neurons and interneurons of the adult cerebral cortex
Section snippets
Experimental procedures
St8siaII and St8siaIV knock out strains (Eckhardt et al., 2000, Angata et al., 2004) were backcrossed with C57BL/6J mice for six generations and mice heterozygous for the mutant polysialyltransferase alleles were interbred to obtain wildtype or knockout offspring (St8siaII−/−, St8siaIV−/−). Of each genotype, five males at the age of 3 month were used in this study. All animal experimentation was conducted in accordance with the European Communities Council Directive of November 24, 1986
Neocortex
In wild type animals PSA expressing cells and PSA-positive neuropil were detected in all regions of the neocortex. As exemplarily shown for the cingulate cortex (Figs. 1A and 2A), PSA-positive cells were particularly abundant in deep layers. Similar to the situation in adult rats (Varea et al., 2005, Varea et al., 2007a) many of the PSA-positive cells displayed multipolar morphologies and neuropil staining was generally more intense in deep layers, specially in the infralimbic and prelimbic
Discussion
The comparative immunohistochemical analysis of residual PSA expression in mice lacking either of the two polysialyltransferases, ST8SiaII or ST8SiaIV, provides a detailed picture of the divergent impact of the two enzymes across different regions of the mature mouse forebrain. In overview, the data demonstrate that both polysialyltransferases contribute to PSA synthesis in the mature brain but their share is clearly different. As assessed before by real time RT-PCR and Western blot analyses of
Acknowledgments
Deutsche ForschungsgemeinschaftDFGHi678/4-1 to H.H. Spanish Ministry of Science and Innovation (MICINN-FEDER)BFU2006-07313/BFI and BFU2009-12284/BFI, Generalitat ValencianaCS2009-AP-127 and ACOMP2009/271 to J.N.; Guirado R. has a FPI predoctoral fellowship from the Spanish Ministry of Education and Science (BES 2007-15757).
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