Clinical NeuroscienceResearch PaperSupplementation of acellular nerve grafts with skin derived precursor cells promotes peripheral nerve regeneration
Section snippets
Cell culture
SKPs were generated from dermis of postnatal day 2 Lewis rats and cultured according to published protocols (Toma et al., 2001). Briefly, pups were quickly decapitated and skin on the dorsal torso was sterilized with a 70% EtOH swab prior to removal with sterile scissors. Collected tissue was minced in Hank's balanced salt solution (HBSS; GIBCO, Burlington, ON, Canada) on ice and then incubated for approximately 45 min in 0.1% collagenase at 37 °C. Skin pieces were mechanically dissociated,
Results
In all animals, the surgical procedures were well-tolerated and wounds healed without complication or signs of pain or discomfort. Graft repair remained intact in every case and there was no evidence of excessive fibrosis in any of the groups.
Discussion
The major findings in this study were (1) Skin-derived precursor cells pre-differentiated towards a SC phenotype in vitro survived, migrated, and maintained the expression of SC markers for at least 8 weeks when transplanted into an acellular (freeze-thawed) isografts (2) After 4 weeks SKP–SCs supported axonal elongation to the same extent as autograft and SC treatment; and (3) provided superior axon regeneration, myelination, and electrophysiological recovery as compared to media control at
Acknowledgments
The authors thank Shahbaz Syed, Joanne Forden and Dr. Qing Gui Xu for their technical expertise and assistance with the preparation of this manuscript. We thank Dr. Freda Miller and her laboratory personnel for demonstrating the techniques of SKP culture and generation. This research was supported by a grant from the Canadian Institutes for Health Research (MOP 82726) and an NSERC CGSD studentship granted to S.K.W. The authors have no conflict of interest.
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2021, Bioactive MaterialsCitation Excerpt :Therefore, ADSCs are regarded as an ideal cell source for transplantation in peripheral nerve regeneration [32]. As for the transplantation procedures, several methods have been adopted, such as (1) directly injecting cell suspension into NGCs [33,34], (2) injecting cell suspension into NGCs followed by in vitro culture [35,36], and (3) mixing cells with Matrigel [14,29] or collagen [27,32] and then injecting them into NGCs. However, each of these methods has its own problems, such as cell leaking, low surviving rate due to lack of substrate during transplantation [37], complicated loading procedures, causing congestion of the NGCs, and risk of immunological reaction.