NeuroanatomyResearch PaperExpression analysis of green fluorescent protein in retinal neurons of four transgenic mouse lines
Section snippets
Generation of transgenic mice
The generation of the PV-EGFP transgenic mice was described in detail by Meyer et al. (2002), the generation of the ChAT-EGFP transgenic mice was described by von Engelhardt et al. (2007), and CR-EGFP transgenic mice were described by Caputi et al. (2008).
5-HTR3A-EGFP transgenic mice were generated using BACs by homologous recombination (Inta et al., 2008). BAC clones spanning the mouse 5-HTR3A gene were identified by polymerase chain reaction screening of libraries of mouse genomic DNA.
Results
In all four transgenic mouse lines a specific expression pattern of EGFP could be observed (Fig. 1). In the mouse line (Fig. 1A), where EGFP expression was under the control of the CR promoter (CR-EGFP mouse) label was confined to amacrine cells of the inner nuclear layer (INL) and to displaced amacrine cells and possibly ganglion cells of the GCL. Two narrow dendritic strata were labeled in the IPL.
The labeling pattern of the ChAT-EGFP mouse (Fig. 1B) was different. Many amacrine cells of the
The BAC technology
Transgenic mice that express reliably and at high level detectable markers such as EGFP in distinct neuronal populations represent an important tool for anatomical and functional studies. The generation of transgenic mice using BACs has become a very useful approach for studying gene expression in vivo in the nervous system (Gong et al., 2003). Generally, BAC transgenes allow the labeling of entire neuronal populations. Nevertheless, they do not guarantee a perfect recapitulation of the
Acknowledgments
We thank B. Marshallsay for excellent technical assistance and I. Odenthal for typing the manuscript.
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