Behavioural neuroscienceActivation of basolateral amygdala corticotropin-releasing factor 1 receptors modulates the consolidation of contextual fear
Section snippets
Experimental animals
Subjects were adult male Long-Evans rats (245–310 g) bred at the University of Hawaii Animal Facility from stock obtained from Charles River Laboratories (Raleigh, NC, USA). Rats were individually housed in polycarbonate cages 1 week prior to the experiments and maintained on a 12-h light/dark schedule with lights on at 06:00 h. Each cage was provisioned with food, water and a layer of Sani-chips. Animal testing occurred between 08:00 and 12:00 h. All procedures were approved by the University
Acquisition test
Rats treated with different doses of DMP696 showed very little freezing during the 2-min preshock interval. However, administration of foot shock increased the duration of contextual freezing across the first three post-shock acquisition intervals, F(5,175)=214.42, P<0.001 (Fig. 1A), and freezing remained at high levels from post-shock intervals 3–5. The overall duration of freezing did not differ significantly in rats treated with different doses of DMP696, F(4,35)=1.56, P>0.05. In addition,
Discussion
The present results demonstrate that CRF1 receptors, and more specifically BLA CRF1 receptors, play an important role in the consolidation of emotional memory. We first showed that oral administration of DMP696, the selective CRF1 receptor antagonist, prior to the acquisition of contextual fear conditioning subsequently produced a dose-dependent reduction in the drug-free conditioned freezing test without significant behavioral effects on the acquisition of contextual freezing. Another study
Conclusion
The present experiments provide new information implicating BLA CRF1 receptors in the consolidation of contextual fear memory. These results expand the list of other neurotransmitter (e.g. norepinephrine, acetylcholine) and hormone (e.g. corticosterone) receptor systems that may have unique or additional cellular actions in BLA cells participating in the consolidation of emotional experiences (McGaugh 2004, Pare 2003, Roozendaal 2000). Importantly, the ability to compromise the consolidation of
Acknowledgments
The research was supported by National Institutes of Health grant NS39406.
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